Generation of Trophoblast Stem Cells from Rabbit Embryonic Stem Cells with BMP4

KIZ OpenIR > 生殖与发育生物学

	Generation of Trophoblast Stem Cells from Rabbit Embryonic Stem Cells with BMP4
	Tan T 1,2,3; Tang XH 1,3; Zhang J 1,2,3; Niu YY 1,3; Chen HW 1,2,3; Li B 1,2,3; Wei QA 1,2,3; Ji WZ*1,3,4; wji@mail.kiz.ac.cn
	2011
发表期刊	PLOS ONE
卷号	6 期号:2 页码:e17124
合作性质	其它
摘要	Trophoblast stem (TS) cells are ideal models to investigate trophectoderm differentiation and placental development. Herein, we describe the derivation of rabbit trophoblast stem cells from embryonic stem (ES) cells. Rabbit ES cells generated in our laboratory were induced to differentiate in the presence of BMP4 and TS-like cell colonies were isolated and expanded. These cells expressed the molecular markers of mouse TS cells, were able to invade, give rise to derivatives of TS cells, and chimerize placental tissues when injected into blastocysts. The rabbit TS-like cells maintained self-renewal in culture medium with serum but without growth factors or feeder cells, whilst their proliferation and identity were compromised by inhibitors of FGFs and TGF-beta receptors. Taken together, our study demonstrated the derivation of rabbit TS cells and suggested the essential roles of FGF and TGF-beta signalings in maintenance of rabbit TS cell self-renewal.
资助者	This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159 ; This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159 ; This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159 ; This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159
收录类别	SCI
语种	英语
资助者	This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159 ; This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159 ; This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159 ; This work was supported by research grants from the Major State Research Development Program 2006CB701505, 2007CB947701; National Basic Research Program 2006AA02A116; Chinese Academy of Sciences KSCX2-YW-R-47; and Chinese National Science Foundation 30570906 and 30700159
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6576
专题	生殖与发育生物学中国科学院昆明灵长类研究中心
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Graduate School of Chinese Academy of Sciences, Beijing, China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming, Yunnan, China 4.Kunming Biomed International, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Tan T,Tang XH,Zhang J,et al. Generation of Trophoblast Stem Cells from Rabbit Embryonic Stem Cells with BMP4[J]. PLOS ONE,2011,6(2):e17124.
APA	Tan T.,Tang XH.,Zhang J.,Niu YY.,Chen HW.,...&wji@mail.kiz.ac.cn.(2011).Generation of Trophoblast Stem Cells from Rabbit Embryonic Stem Cells with BMP4.PLOS ONE,6(2),e17124.
MLA	Tan T,et al."Generation of Trophoblast Stem Cells from Rabbit Embryonic Stem Cells with BMP4".PLOS ONE 6.2(2011):e17124.

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