Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera

	Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera
	Xu PJ 1,4; Li T 1,4; Xiao JH 1; Murphy RW 3,5; Huang DW*1,2; huangdw@ioz.ac.cn
	2011
发表期刊	EUROPEAN JOURNAL OF ENTOMOLOGY
卷号	108 期号:1 页码:164-168
合作性质	其它
摘要	Using sequence alignment, a conserved domain in the 3' untranslated region (UTR) of the cytoplasmic heat shock protein 90 (HSP90) of Lepidoptera was found. This region is highly variable in other insect groups. Furthermore, universal primers were designed to amplify the complete coding sequence (CDS) of HSP90 from total genomic DNA in Lepidoptera, avoiding the commonly used reverse transcription-polymerase chain reaction (RT-PCR) and 3', 5'-rapid amplification of cDNA ends (RACE) methods based on cDNA. These primers amplified a fragment of about 2.25 kb in the 11 species tested, which represent seven different families of Lepidoptera, including moths and butterflies. The results suggest that the conserved domain of 3' UTR is universal in Lepidoptera and these primers successfully amplify the complete CDS of cytoplasmic HSP90 from genomic DNA.
关键词	Coding Sequence Untranslated Region Hsp90 Race Universal Primer Lepidoptera
资助者	This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). ; This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). ; This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). ; This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109).
收录类别	SCI
语种	英语
资助者	This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). ; This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). ; This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109). ; This project was supported by the National Natural Science Foundation of China (NSFC grant no. 30770302, 30570970), partially by Program of Ministry of Sci- ence and Technology of the Republic of China (2006FY110500) and by National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC- J0630964/J0109).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6585
专题	科研部门_分子进化与基因组多样性（张亚平）遗传资源与进化国家重点实验室
通讯作者	huangdw@ioz.ac.cn
作者单位	1.Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China 2.College of Life Sciences, Hebei University, Baoding, 071002, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Graduate School of the Chinese Academy of Sciences, Beijing, China 5.Department of Natural History, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada
推荐引用方式 GB/T 7714	Xu PJ,Li T,Xiao JH,et al. Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera[J]. EUROPEAN JOURNAL OF ENTOMOLOGY,2011,108(1):164-168.
APA	Xu PJ,Li T,Xiao JH,Murphy RW,Huang DW*,&huangdw@ioz.ac.cn.(2011).Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera.EUROPEAN JOURNAL OF ENTOMOLOGY,108(1),164-168.
MLA	Xu PJ,et al."Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera".EUROPEAN JOURNAL OF ENTOMOLOGY 108.1(2011):164-168.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2011031601.pdf（872KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文