A Novel Enzyme-Linked Immunosorbent Assay for Screening HIV-1 Fusion Inhibitors Targeting HIV-1 Gp41 Core Structure

	A Novel Enzyme-Linked Immunosorbent Assay for Screening HIV-1 Fusion Inhibitors Targeting HIV-1 Gp41 Core Structure
	Pang W 1; Wang RR 1; Gao YD 2; Yang LM 1; Sun Y 1; Huang JF 2; Tien P 3; Zheng YT*1; zhengyt@mail.kiz.ac.cn
	2011
发表期刊	JOURNAL OF BIOMOLECULAR SCREENING
卷号	16 期号:2 页码:221-229
合作性质	其它
摘要	The gp41 subunit of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein mediates the fusion of viral and host cell membranes. As the HIV-1 enters the host cells, the 2 helical regions, HR1 and HR2, in the ectodomain of gp41 can form a 6-helix bundle, which brings the viral and target cell membranes to close proximity and serves as an attractive target for developing HIV-1 fusion inhibitors. Now, there are several cell-and molecule-based assays to identify potential HIV-1 fusion inhibitors targeting gp41. However, these assays cannot be used universally because they are time-consuming, inconvenient, and expensive. In the present study, the authors expressed and purified GST-HR121 and C43-30a proteins that were derived from the HIV-1 gp41 ectodomain region. GST-HR121 has a function similar to the HR1 peptide of gp41, whereas C43-30a is an HR2-derived peptide that added 50 amino acid residues (aa) in the N-terminal of C43. Further research found they could interact with each other, and a potential HIV-1 fusion inhibitor could inhibit this interaction. On the basis of this fact, a novel, rapid, and economic enzyme-linked immunosorbent assay was established, which can be developed for high-throughput screening of HIV-1 fusion inhibitors.
关键词	Hiv-1 Fusion Inhibitor Enzyme-linked Immunosorbent Assay Gst-hr121 C34-30a
资助者	this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601). ; this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601). ; this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601). ; this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601).
收录类别	SCI
语种	英语
资助者	this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601). ; this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601). ; this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601). ; this work was supported in part by grants from the chinese academy of sciences (KscX1- yW-r-24, KscX2-yW-r-185), eleventh five-year Key scientific and technological program of china (2009ZX09501- 029, 2008ZX10001-002, 2008ZX10001-015, 2008ZX10005-005) and yunnan province (2007bc006, 2009cd109), national basic research program of china (2009cb5223006), china postdoctoral science foundation (20090451433), and the national natural science foundation of china (u0832601).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6586
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室遗传资源与进化国家重点实验室结构生物信息学管理、支撑部门_大型仪器中心
通讯作者	zhengyt@mail.kiz.ac.cn
作者单位	1.Key Laboratory of animal models and human disease mechanisms of chinese academy of sciences and yunnan province, Kunming institute of Zoology, chinese academy of sciences, Kunming, china 2.State Key Laboratory of genetic resources and evolution, Kunming institute of Zoology, chinese academy of sciences, Kunming, china. 3.center for molecular virology, institute of microbiology, chinese academy of sciences, beijing, china.
推荐引用方式 GB/T 7714	Pang W,Wang RR,Gao YD,et al. A Novel Enzyme-Linked Immunosorbent Assay for Screening HIV-1 Fusion Inhibitors Targeting HIV-1 Gp41 Core Structure[J]. JOURNAL OF BIOMOLECULAR SCREENING,2011,16(2):221-229.
APA	Pang W.,Wang RR.,Gao YD.,Yang LM.,Sun Y.,...&zhengyt@mail.kiz.ac.cn.(2011).A Novel Enzyme-Linked Immunosorbent Assay for Screening HIV-1 Fusion Inhibitors Targeting HIV-1 Gp41 Core Structure.JOURNAL OF BIOMOLECULAR SCREENING,16(2),221-229.
MLA	Pang W,et al."A Novel Enzyme-Linked Immunosorbent Assay for Screening HIV-1 Fusion Inhibitors Targeting HIV-1 Gp41 Core Structure".JOURNAL OF BIOMOLECULAR SCREENING 16.2(2011):221-229.

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