A novel natriuretic peptide from the cobra venom

	A novel natriuretic peptide from the cobra venom
	Zhang Yong 1; Wu JB 1; Yu GY 1,2; Chen ZM 1; Zhou XD 1; Zhu SW 1; Li R 1; Zhang Y 1; Lu QM*1; lvqm@mail.kiz.ac.cn
	2011
发表期刊	TOXICON
卷号	57 期号:1 页码:134-140
合作性质	其它
摘要	Natriuretic peptides (NPs) play crucial roles in human physiology and pathophysiology through natriuresis, dieresis and vasorelaxation. NPs are also one of the important components of snake venoms. However, the low abundance in snake venom hampered the investigation. Here, a novel natriuretic peptide named Na-NP was purified from the cobra Naja atra venom. Na-NP consists of 45 amino acid residues and its molecular weight is 4618.5 Da. A full-length cDNA encoding Na-NP was obtained from the cDNA library constructed from the venom gland. The open reading frame of cloned Na-NP was composed of 498 bp and coded for a 165-amino acid residue protein precursor. The nucleotide and deduced protein sequences of Na-NP were remarkably conserved with other elapid NPs while significant different from the viperid NPs. Na-NP showed weak activity to relax the aortic rings pre-contracted with phenylephrine. Meanwhile, Na-NP showed cGMP-promotion activity against primary cultured rabbit endocardial endothelial cells, but had no effect on human platelet aggregation. In conclusion, this is the first report of a natriuretic peptide from the cobra N. atra venom. Na-NP might be served as a useful tool for the study of human NPs and the development of novel therapeutic drugs. (C) 2010 Elsevier Ltd. All rights reserved
关键词	Natriuretic Peptide Elapid Snake Purification Molecular Cloning
资助者	This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M).
收录类别	SCI
语种	英语
资助者	This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6590
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室科研部门_生物毒素与人类疾病（张云）其他
通讯作者	lvqm@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Kunming Med Coll, Dept Biochem, Kunming 650032, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhang Yong,Wu JB,Yu GY,et al. A novel natriuretic peptide from the cobra venom[J]. TOXICON,2011,57(1):134-140.
APA	Zhang Yong.,Wu JB.,Yu GY.,Chen ZM.,Zhou XD.,...&lvqm@mail.kiz.ac.cn.(2011).A novel natriuretic peptide from the cobra venom.TOXICON,57(1),134-140.
MLA	Zhang Yong,et al."A novel natriuretic peptide from the cobra venom".TOXICON 57.1(2011):134-140.

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