Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)

	Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)
	Ng YM 1; Yang YH 1; Sze KH 2; Zhang X 3,4; Zheng YT 3; Shaw PC*1; pcshaw@cuhk.edu.hk
	2011
发表期刊	JOURNAL OF STRUCTURAL BIOLOGY
卷号	174 期号:1 页码:164-172
合作性质	其它
摘要	Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif
关键词	Luffin P1 Ribosome-inactivating Peptide Nuclear Magnetic Resonance Anti-hiv-1
资助者	The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). ; The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). ; The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). ; The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185).
收录类别	SCI
语种	英语
资助者	The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). ; The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). ; The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185). ; The work in Hong Kong was supported by grants from the Re- search Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Ying- hua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equip- ment Grant (SEG CUHKØ8 and CUHKØ9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Techno- logical Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R- 185).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6603
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	pcshaw@cuhk.edu.hk
作者单位	1.School of Life Sciences and Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, PR China 2.Chemistry Department and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China 3.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Ng YM,Yang YH,Sze KH,et al. Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)[J]. JOURNAL OF STRUCTURAL BIOLOGY,2011,174(1):164-172.
APA	Ng YM.,Yang YH.,Sze KH.,Zhang X.,Zheng YT.,...&pcshaw@cuhk.edu.hk.(2011).Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica).JOURNAL OF STRUCTURAL BIOLOGY,174(1),164-172.
MLA	Ng YM,et al."Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)".JOURNAL OF STRUCTURAL BIOLOGY 174.1(2011):164-172.

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