Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors

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	Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors
	Bao L 1; He LXZ 1; Chen JJ 1; Wu Z 1; Liao J 1; Rao LJ 1; Ren JT 1; Li H 3; Zhu H 1; Qian L 1; Gu YJ 1; Dai HM 1; Xu X 5; Zhou JQ 2; Wang W 5; Cui C 1,4; Xiao L*1,4; leixiao@sibs.ac.cn, leixiao@zju.edu.cn
	2011
发表期刊	CELL RESEARCH
卷号	21 期号:4 页码:600-608
合作性质	其它
摘要	Reprogramming of somatic cells in the enucleated egg made Dolly, the sheep, the first successfully cloned mammal in 1996. However, the mechanism of sheep somatic cell reprogramming has not yet been addressed. Moreover, sheep embryonic stem (ES) cells are still not available, which limits the generation of precise gene-modified sheep. In this study, we report that sheep somatic cells can be directly reprogrammed to induced pluripotent stem (iPS) cells using defined factors (Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 large T and hTERT). Our observations indicated that somatic cells from sheep are more difficult to reprogram than somatic cells from other species, in which iPS cells have been reported. We demonstrated that sheep iPS cells express ES cell markers, including alkaline phosphatase, Oct4, Nanog, Sox2, Rex1, stage-specific embryonic antigen-1, TRA-1-60, TRA-1-81 and E-cadherin. Sheep iPS cells exhibited normal karyotypes and were able to differentiate into all three germ layers both in vitro and in teratomas. Our study may help to reveal the mechanism of somatic cell reprogramming in sheep and provide a platform to explore the culture conditions for sheep ES cells. Moreover, sheep iPS cells may be directly used to generate precise gene-modified sheep.
关键词	Sheep Pluripotency Reprogram Ips Cells Embryonic Stem Cells
资助者	This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223). ; This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223). ; This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223). ; This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223).
收录类别	SCI
语种	英语
资助者	This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223). ; This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223). ; This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223). ; This research was partially supported by grants from the National Key Basic Research and Development Program of China (2007CB947902, 2007CB948003, 2009CB941101, 2009CB940903), the National Natural Science Foundation of China (30971682, 30901019, 31025016), the Ministry of Agri- culture (2009ZX08010-016B), the National High Technology Research and Development Program (863 program) of China (2010AA100504) and the Chinese Academy of Sciences Knowl- edge Creation Program (KSCX2-YW-R-223).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6610
专题	基因起源组遗传资源与进化国家重点实验室
通讯作者	leixiao@sibs.ac.cn, leixiao@zju.edu.cn
作者单位	1.Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 2.The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 3.Xiangtan Center Hospital, Hunan 411100, China 4.College of Animal Sciences, Zhejiang University, Hangzhou 310058, China 5.CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Bao L,He LXZ,Chen JJ,et al. Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors[J]. CELL RESEARCH,2011,21(4):600-608.
APA	Bao L.,He LXZ.,Chen JJ.,Wu Z.,Liao J.,...&leixiao@sibs.ac.cn, leixiao@zju.edu.cn.(2011).Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors.CELL RESEARCH,21(4),600-608.
MLA	Bao L,et al."Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors".CELL RESEARCH 21.4(2011):600-608.

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