| Cloning and sequence analysis of an Ophiophagus hannah cDNA encoding a precursor of two natriuretic pepide domains | |
| Lei WW1,2; Zhang Yong*1; Yu GY1,3; Jiang P1; He YY1; Lee WH1; Zhang Y*1; zhyong@mail.kiz.ac.cn | |
| 2011 | |
| 发表期刊 | TOXICON
 |
| 卷号 | 57期号:5页码:811-816 |
| 合作性质 | 其它 |
| 摘要 | The king cobra (Ophiophagus hannah) is the largest venomous snake. Despite the components are mainly neurotoxins, the venom contains several proteins affecting blood system. Natriuretic peptide (NP), one of the important components of snake venoms, could cause local vasodilatation and a promoted capillary permeability facilitating a rapid diffusion of other toxins into the prey tissues. Due to the low abundance, it is hard to purify the snake venom NPs. The cDNA cloning of the NPs become a useful approach. In this study, a 957 bp natriuretic peptide-encoding cDNA clone was isolated from an O. hannah venom gland cDNA library. The open-reading frame of the cDNA encodes a 210-amino acid residues precursor protein named Oh-NP. Oh-NP has a typical signal peptide sequence of 26 amino acid residues. Surprisingly, Oh-NP has two typical NP domains which consist of the typical sequence of 17-residue loop of CFGXXDRIGC, so it is an unusual NP precursor. These two NP domains share high amino acid sequence identity. In addition, there are two homologous peptides of unknown function within the Oh-NP precursor. To our knowledge, Oh-NP is the first protein precursor containing two NP domains. It might belong to another subclass of snake venom NPs |
| 关键词 | Natriuretic Peptide Elapid Snake Molecular Cloning Sequence Analysis |
| 资助者 | .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). ; .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). ; .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). ; .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). ; .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). ; .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). ; .ThisworkwassupportedbygrantsfromNational Basic Research Program of China (973 Program, 2010CB 529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese AcademyofSciences“KeyResearchDirection”(KSCX2-YW-R- 088) and “Western Light Project” (Y102291081). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/353002/6620 |
| 专题 | 科研部门_生物毒素与人类疾病（张云） 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | zhyong@mail.kiz.ac.cn |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, Kunming 650223, China 2.Laboratory of Molecular Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China 3.Department of Biochemistry, Kunming Medical College, Yunnan, Kunming 650032, China |
| 推荐引用方式 GB/T 7714 | Lei WW,Zhang Yong*,Yu GY,et al. Cloning and sequence analysis of an Ophiophagus hannah cDNA encoding a precursor of two natriuretic pepide domains[J]. TOXICON,2011,57(5):811-816. |
| APA | Lei WW.,Zhang Yong*.,Yu GY.,Jiang P.,He YY.,...&zhyong@mail.kiz.ac.cn.(2011).Cloning and sequence analysis of an Ophiophagus hannah cDNA encoding a precursor of two natriuretic pepide domains.TOXICON,57(5),811-816. |
| MLA | Lei WW,et al."Cloning and sequence analysis of an Ophiophagus hannah cDNA encoding a precursor of two natriuretic pepide domains".TOXICON 57.5(2011):811-816. |
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| 2011061302.pdf（1073KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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