Rapid identification of mtDNA somatic mutations in gastric cancer tissues based on the mtDNA phylogeny

KIZ OpenIR > 科研部门 > 疾病机理遗传学和进化医学学科组（姚永刚）

	Rapid identification of mtDNA somatic mutations in gastric cancer tissues based on the mtDNA phylogeny
	Bi R 1,3; Li WL 2; Chen MQ 2; Zhu Z 2; Yao YG*1; ygyaozh@gmail.com
	2011
发表期刊	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
卷号	709-710 期号:X 页码:15-20
摘要	Mitochondrial DNA (mtDNA) somatic mutations have been identified in nearly all kinds of cancer during the past decade. Normally one need to determine the complete mtDNA sequences from both cancerous and normal tissues of the same patient to score the somatic mutation in cancer. In this study, we intended to explore a strategy to quickly identify somatic mutations in the entire mtDNA genome based on its phylogeny. The principal assumption for this strategy is that somatic mutations, as recently accumulated in cancerous tissue, have younger age and will be located in the terminal branches of mtDNA phylogenetic tree. In contrast, the haplogroup-specific variants, which appear as germline variants and have ancient age, will be located in the basal or intermediate-node branches of the tree, depending on their relative age. When the complete mtDNA sequence of the cancerous tissue is determined and is classified relative to the available mtDNA phylogeny, we only need to screen the variants that are located in the terminal branch in the paracancerous tissue or other normal tissue from the same patient to identify somatic mutations in cancer. We validated this strategy by using paired gastric cancer tissue and paracancerous tissue or blood from 10 Chinese patients (including one with gastric stromal tumor). A total of seven somatic mutations were identified in the cancerous tissues from four patients. Our result suggests that employing mtDNA phylogenetic knowledge facilitates rapid identification of mitochondrial genome somatic mutations in cancer
关键词	Mtdna Somatic Mutations Cancer Phylogeny
资助者	This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900). ; This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900). ; This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900). ; This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900).
收录类别	SCI
语种	英语
资助者	This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900). ; This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900). ; This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900). ; This study was supported by Yunnan Province ( 2009CI119), the National Natural Science FoundationofChina(30925021),andtheMOST(MinistryofScience and Technology of China; 2011CB910900).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6623
专题	科研部门_疾病机理遗传学和进化医学学科组（姚永刚）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	ygyaozh@gmail.com
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, 32 Jiaochang Donglu, Kunming, Yunnan 650223, China 2.Department of Oncology, the First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Bi R,Li WL,Chen MQ,et al. Rapid identification of mtDNA somatic mutations in gastric cancer tissues based on the mtDNA phylogeny[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2011,709-710(X):15-20.
APA	Bi R,Li WL,Chen MQ,Zhu Z,Yao YG*,&ygyaozh@gmail.com.(2011).Rapid identification of mtDNA somatic mutations in gastric cancer tissues based on the mtDNA phylogeny.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,709-710(X),15-20.
MLA	Bi R,et al."Rapid identification of mtDNA somatic mutations in gastric cancer tissues based on the mtDNA phylogeny".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 709-710.X(2011):15-20.

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