beta gamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex, induces calcium-dependent platelet apoptosis

	beta gamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex, induces calcium-dependent platelet apoptosis
	Gao Q 1,2; Xiang Y 1,3; Chen ZM 1,3; Zeng L 1,3; Ma XT 1,3; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2011
发表期刊	THROMBOSIS AND HAEMOSTASIS
卷号	105 期号:5 页码:846-854
合作性质	其它
摘要	In recent years, it has been reported that apoptosis may occur in platelets and play a role in the clearance of effete platelets. beta gamma-CAT, a newly identified non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions, caused several in vivo toxic effects on mammals. Through determined haematological parameters of rabbits, it has been found that beta gamma-CAT significantly reduced the number of platelets in a time-dependent manner. Here, in order to explore the effect of beta gamma-CAT on platelets, washed platelets were incubated with various concentrations of beta gamma-CAT for 30 minutes.We found that beta gamma-CAT induced several apoptosis events in human platelets, including caspase-3 activation, phosphatidylserine (PS) exposure, depolarisation of mitochondrial inner transmembrane potential (Delta Psi m), cytochrome c release and strong expression of pro-apoptotic Bax and Bak proteins. However, beta gamma-CAT did not significantly induce platelet activation as detected by P-selectin surface expression, GPIlb/Illa activation and platelet aggregation. In addition, we observed that beta gamma-CAT-induced PS exposure and Delta Psi m depolarisation in platelets are Ca(2+)-dependent. Taken together, beta gamma-CAT can induce Ca(2+)-dependent platelet apoptosis but does not cause platelet activation.
关键词	Platelet Apoptosis Non-lens Beta Gamma-crystallins Trefoil Factor Beta Gamma-cat Ca(2+)
资助者	This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406). ; This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406). ; This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406). ; This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406).
收录类别	SCI
语种	英语
资助者	This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406). ; This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406). ; This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406). ; This study was supported by National Nature Science Foundation of China (81072162, 81120108019, and U1132605) , Top Talents Program of Yunnan Province, China (2010CI114), and Strategic Priority Research Program of the Chinese Academy of Sciences，Stem Cell and Regenerative Medicine Research (XDA01040406).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6656
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.School of Life Science, University of Science and Technology of China, Hefei, China 3.Graduate School of Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Gao Q,Xiang Y,Chen ZM,et al. beta gamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex, induces calcium-dependent platelet apoptosis[J]. THROMBOSIS AND HAEMOSTASIS,2011,105(5):846-854.
APA	Gao Q.,Xiang Y.,Chen ZM.,Zeng L.,Ma XT.,...&zhangy@mail.kiz.ac.cn.(2011).beta gamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex, induces calcium-dependent platelet apoptosis.THROMBOSIS AND HAEMOSTASIS,105(5),846-854.
MLA	Gao Q,et al."beta gamma-CAT, a non-lens betagamma-crystallin and trefoil factor complex, induces calcium-dependent platelet apoptosis".THROMBOSIS AND HAEMOSTASIS 105.5(2011):846-854.

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