Hypomethylation of the HTR1A promoter region and high expression of HTR1A in the peripheral blood lymphocytes of patients with systemic lupus erythematosus

	Hypomethylation of the HTR1A promoter region and high expression of HTR1A in the peripheral blood lymphocytes of patients with systemic lupus erythematosus
	Xu J 1; Zhang GL 2; Cheng YQ 3,4; Chen B 2; Dong Y 3; Li LQ 1; Xu L 4,5; Xu XQ 3; Lu ZP 1; Wen JF*2; wenjf@mail.kiz.ac.cn
	2011
发表期刊	LUPUS
卷号	20 期号:7 页码:678-689
合作性质	其它
摘要	The occurrence of systemic lupus erythematosus (SLE) involves a gene-environment interaction and epigenetic regulations, such as DNA methylation, may play important role in the etiology of SLE. Some neurotransmitters, such as serotonin, can regulate T- and B-cell proliferation via the 5-HT1A receptor and are involved in the pathology of SLE. The abnormal methylation of DNA has been reported in SLE, but there has been no study concerning the serotonin system. This study was conducted to explore the DNA methylation status of the promoter region of HTR1A (PR-HTR1A) and the level of HTR1A mRNA in the peripheral blood lymphocytes (PBLC) of SLE patients and healthy controls (HC). In this study, the DNA methylation status of PR-HTR1A and the level of HTR1A mRNA were detected in the PBLC of SLE patients and HC. The results showed significant hypomethylation of PR-HTR1A in SLE patients compared with HC. The patients also showed a significantly higher HTR1A mRNA level than did the controls. Relatively higher percentage of anti-histone antibodies in methylated SLE patients was found compared with unmethylated patients. Our results support the hypothesis that the hypomethylation of PR-HTR1A and overexpression of HTR1A might contribute to SLE. These results also reveal that epigenetic regulation via the serotonin system may contribute to SLE, and reveal the link between the brain and the immune system.
关键词	Depression Dna Methylation Htr1a Gene Promoter Region Sle
资助者	This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX). ; This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX). ; This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX). ; This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX).
收录类别	SCI
语种	英语
资助者	This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX). ; This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX). ; This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX). ; This work is supported by the United Foundation of the 973 Program (grant number 2007CB815705), Yunnan province (grant number 2008GA002 to JW), the National Natural Science Foundation of China (grant numbers 30830018 and 30623007 to JW), the Yunnan Provincial Science and Technology Department and Kunming Medical College (grant number 2008C0005R to XFX), the National Natural Science Foundation of China (30530250 to LX), the Science and Technology Foundation of Yunnan Province (grant number 2006PT08 to LX), and the 973 Program from the Ministry of Science and Technology of China (grant numbers 2006CB500808 and 2009CB941300 to LX).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6657
专题	科研部门_真核细胞进化基因组（文建凡）科研部门_动物模型与人类重大疾病机理重点实验室遗传资源与进化国家重点实验室科研部门_学习记忆的分子神经机制（徐林）
通讯作者	wenjf@mail.kiz.ac.cn
作者单位	1.Department of Rheumatology and Immunology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, PR China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China and Graduate School of Chinese Academy of Sciences, Beijing, PR China 3.Department of Psychiatry, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, PR China 4.Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, PR China and Graduate School of Chinese Academy of Sciences, Beijing, PR China 5.Mental Health Institute, the 2nd Hospital of Xiangya Medical College, Central South University, Changsha, PR China
推荐引用方式 GB/T 7714	Xu J,Zhang GL,Cheng YQ,et al. Hypomethylation of the HTR1A promoter region and high expression of HTR1A in the peripheral blood lymphocytes of patients with systemic lupus erythematosus[J]. LUPUS,2011,20(7):678-689.
APA	Xu J.,Zhang GL.,Cheng YQ.,Chen B.,Dong Y.,...&wenjf@mail.kiz.ac.cn.(2011).Hypomethylation of the HTR1A promoter region and high expression of HTR1A in the peripheral blood lymphocytes of patients with systemic lupus erythematosus.LUPUS,20(7),678-689.
MLA	Xu J,et al."Hypomethylation of the HTR1A promoter region and high expression of HTR1A in the peripheral blood lymphocytes of patients with systemic lupus erythematosus".LUPUS 20.7(2011):678-689.

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