| Functional Evolution of BRCT Domains from Binding DNA to Protein | |
| Sheng ZZ1,2; Zhao YQ1,2; Huang JF*1,3; huangjf@mail.kiz.ac.cn | |
| 2011 | |
| 发表期刊 | EVOLUTIONARY BIOINFORMATICS
 |
| 卷号 | 7期号:X页码:87-97 |
| 合作性质 | 其它 |
| 摘要 | The BRCT domain (BRCA1 C-terminal domain) is an important signaling and protein targeting motif in the DNA damage response system. The BRCT domain, which mainly occurs as a singleton (single BRCT) or tandem pair (double BRCT), contains a phosphate-binding pocket that can bind the phosphate from either the DNA end or a phosphopeptide. In this work, we performed a database search, phylogeny reconstruction, and phosphate-binding pocket comparison to analyze the functional evolution of the BRCT domain. We identified new BRCT-containing proteins in bacteria and eukaryotes, and found that the number of BRCT-containing proteins per genome is correlated with genome complexity. Phylogeny analyses revealed that there are two groups of single BRCT domains (sGroup I and sGroup II) and double BRCT domains (dGroup I and dGroup II). These four BRCT groups differ in their phosphate-binding pockets. In eukaryotes, the evolution of the BRCT domain can be divided into three phases. In the first phase, the sGroup I BRCT domain with the phosphate-binding pocket that can bind the phosphate of nicked DNA invaded eukaryotic genome. In the second phase, the phosphate-binding pocket changed from a DNA-binding type to a protein-binding type in sGroup II. The tandem duplication of sGroup II BRCT domain gave birth to double BRCT domain, from which two structurally and functionally distinct groups were evolved. The third phase is after the divergence between animals and plants. Both sGroup I and sGroup II BRCT domains originating in this phase lost the phosphate-binding pocket and many evolved protein-binding sites. Many dGroup I members were evolved in this stage but few dGroup II members were observed. The results further suggested that the BRCT domain expansion and functional change in eukaryote may be driven by the evolution of the DNA damage response system. |
| 关键词 | Brct Domain Evolution Dna Damage Response Superfamily |
| 资助者 | This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). ; This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). ; This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). ; This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). ; This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). ; This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). ; This work was supported by Grants to JFH from the National Basic Research Program of China (Grant No. 2007CB815705), the National Natural Sci- ence Foundation of China (Grant No. 30623007 and 30621092), and Chinese Academy of Sciences (Grant No. 2007211311091). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/353002/6715 |
| 专题 | 结构生物信息学 遗传资源与进化国家重点实验室 |
| 通讯作者 | huangjf@mail.kiz.ac.cn |
| 作者单位 | 1.State Key Laboratory of genetic resources and Evolution, Kunming institute of Zoology, chinese Academy of Sciences, Kunming, china 2.Graduate School of chinese Academy of Sciences, Beijing, china 3.Kunming institute of Zoology, chinese University of hong Kong Joint research center for Bioresources and human Disease Mechanisms, Kunming, Peoples’ republic of china |
| 推荐引用方式 GB/T 7714 | Sheng ZZ,Zhao YQ,Huang JF*,et al. Functional Evolution of BRCT Domains from Binding DNA to Protein[J]. EVOLUTIONARY BIOINFORMATICS,2011,7(X):87-97. |
| APA | Sheng ZZ,Zhao YQ,Huang JF*,&huangjf@mail.kiz.ac.cn.(2011).Functional Evolution of BRCT Domains from Binding DNA to Protein.EVOLUTIONARY BIOINFORMATICS,7(X),87-97. |
| MLA | Sheng ZZ,et al."Functional Evolution of BRCT Domains from Binding DNA to Protein".EVOLUTIONARY BIOINFORMATICS 7.X(2011):87-97. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 2011082605.pdf（1284KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论