Decreased expression of protease-activated receptor 4 in human gastric cancer

	Decreased expression of protease-activated receptor 4 in human gastric cancer
	Zhang Y 1; Yu GY 1,2; Jiang P 1,3; Xiang Y 1; Li WL 4; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2011
发表期刊	INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
卷号	43 期号:9 页码:1277-1283
合作性质	其它
摘要	Protease-activated receptors (PARs) are a unique family of G-protein coupled receptors. PAR4, the most recently identified PAR member, was reported to be overexpressed during the progression of colon and prostate cancers. Though PAR4 mRNA was detected in normal stomach, the role of PAR4 in gastric cancer has not been investigated. In this study, differential expression of PAR4 was measured by real-time PCR (n = 28) and tissue microarrays (n = 74). We showed that PAR4 was located from basal to middle portions of normal gastric mucosa. PAR4 expression was remarkably decreased in gastric cancer tissues as compared with matched noncancerous tissues, especially in positive lymph node or low differentiation cancers. Furthermore, methylation of the PAR4 promoter in cell lines was assessed by treatment with 5-aza-2'-deoxycytidine and genomic bisulfite sequencing. AGS and N87 human gastric cancer cell lines did not express PAR4, as compared to HT-29 human colon cancer cell line with significant PAR4 expression. Treatment with 5-aza-2'-deoxycytidine restored PAR4 expression in AGS and N87 cells, which exhibited significantly more 5-methylcytosines in the PAR4 promoter compared with HT-29 cells. Our results revealed that down-regulation of PAR4 expression occurs frequently in gastric cancers and exhibits association with more aggressive gastric cancer. Interestingly, the loss of PAR4 expression in gastric cancers may result from hypermethylation of the PAR4 promoter.
关键词	Protease-activated Receptor Gastric Cancer Real-time Pcr Immunohistochemistry Methylation
资助者	This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). ; This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). ; This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). ; This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). ; This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). ; This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081). ; This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30570359 and 30870304), and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088) and “Western Light Project” (Y102291081).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6751
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Department of Biochemistry, Kunming Medical College, Kunming, Yunnan 650032, China 3.Department of Pathology, Kunming Medical College, Kunming, Yunnan 650032, China 4.Department of Gastroenterology, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, China
推荐引用方式 GB/T 7714	Zhang Y,Yu GY,Jiang P,et al. Decreased expression of protease-activated receptor 4 in human gastric cancer[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2011,43(9):1277-1283.
APA	Zhang Y.,Yu GY.,Jiang P.,Xiang Y.,Li WL.,...&zhangy@mail.kiz.ac.cn.(2011).Decreased expression of protease-activated receptor 4 in human gastric cancer.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,43(9),1277-1283.
MLA	Zhang Y,et al."Decreased expression of protease-activated receptor 4 in human gastric cancer".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 43.9(2011):1277-1283.

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