COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)

KIZ OpenIR > 遗传资源与进化国家重点实验室 > 分子进化与基因组多样性（张亚平）

	COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)
	Xia Y 1,2; Gu HF 1,2; Peng R 1; Chen Q 1; Zheng YC 1; Murphy RW 3,4; Zeng XM*1; zengxm@cib.ac.cn
	2012
发表期刊	MOLECULAR ECOLOGY RESOURCES
卷号	12 期号:1 页码:48-56
合作性质	其它
摘要	The 5' region of the mitochondrial DNA (mtDNA) gene cytochrome c oxidase I (COI) is the standard marker for DNA barcoding. However, because COI tends to be highly variable in amphibians, sequencing is often challenging. Consequently, another mtDNA gene, 16S rRNA gene, is often advocated for amphibian barcoding. Herein, we directly compare the usefulness of COI and 16S in discriminating species of hynobiid salamanders using 130 individuals. Species identification and classification of these animals, which are endemic to Asia, are often based on morphology only. Analysis of Kimura 2-parameter genetic distances (K2P) documents the mean intraspecific variation for COI and 16S rRNA genes to be 1.4% and 0.3%, respectively. Whereas COI can always identify species, sometimes 16S cannot. Intra- and interspecific genetic divergences occasionally overlap in both markers, thus reducing the value of a barcoding gap to identify genera. Regardless, COI is the better DNA barcoding marker for hynobiids. In addition to the comparison of two potential markers, high levels of intraspecific divergence in COI (>5%) suggest that both Onychodactylus fischeri and Salamandrella keyserlingii might be composites of cryptic species.
关键词	China Cryptic Species Japan South Korea Species Identification
资助者	This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). ; This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). ; This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). ; This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148).
收录类别	SCI
语种	英语
资助者	This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). ; This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). ; This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148). ; This research was supported by the National Natural Sciences Foundation of China (NSFC-30870287 and NSFC-30900134) and by the Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22 and KSCX2-YW-Z-0906). Manu- script preparation was supported, in part, by the Natural Sciences and Engineering Research Council of Canada (A3148).
WOS记录号	WOS:000298585000005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6895
专题	遗传资源与进化国家重点实验室_分子进化与基因组多样性（张亚平）遗传资源与进化国家重点实验室
通讯作者	zengxm@cib.ac.cn
作者单位	1.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2.Graduate University of Chinese Academy of Sciences, Beijing, 100049, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada
推荐引用方式 GB/T 7714	Xia Y,Gu HF,Peng R,et al. COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)[J]. MOLECULAR ECOLOGY RESOURCES,2012,12(1):48-56.
APA	Xia Y.,Gu HF.,Peng R.,Chen Q.,Zheng YC.,...&zengxm@cib.ac.cn.(2012).COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae).MOLECULAR ECOLOGY RESOURCES,12(1),48-56.
MLA	Xia Y,et al."COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae)".MOLECULAR ECOLOGY RESOURCES 12.1(2012):48-56.

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