KMS KUNMING INSTITUTE OF ZOOLOGY.CAS
| Formation and characteristics of an unusual lambda-DNA species | |
| Cao EH*1; Sun XG1; Wang XW2; Fang Y2; Bai CL2; Liu CQ3; He YJ1 | |
| 1997 | |
| 发表期刊 | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
 |
| 卷号 | 43期号:3页码:541-550 |
| 合作性质 | 其它 |
| 摘要 | An unusual DNA species, termed as DNA species A, has been isolated and purified from thermal-denatured lambda-DNA Hind III by Sephadex G-200 gel filtration. Our studies indicate that DNA species A is resistant to DNase I digestion and has a higher melting point. The new DNA species showed a lower absorbency at 260 nm, and a lower fluorescence quantum yield after interaction with ethidium bromide (EB) than native double-stranded lambda-DNA. CD spectrum of DNA species A consists of a broad positive band centered at 245 nm and a weak negative band at 220 nm. transmission electron microscope (TEM) visualizations showed that their lengths of DNA species A fell mainly in three regions (300-500 nm, 750-1000 nm and 1500 nm) that corresponded to three fluorescence bands in the EB-stained gels. Their apparent width and height were 65-75 nm and 2.2 nm respectively as observed by images of atomic force microscope (AFM). |
| 关键词 | Lambda-dna Triple-stranded Dna Melting Temperature Cd Spectra Fluorescence Spectrum Transmission Electron Microscope Atomic Force Microscope |
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| 收录类别 | 其他 |
| 语种 | 英语 |
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| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/10920 |
| 专题 | 其他 |
| 作者单位 | 1.Institute of Biophysics, Academia Sinica, Beijing, 100101 China 2.Institute of chemistry, Academia Sinica, Beijing, 100080, China 3.Kunming Institute of Zoology, Academia Sinica, Kunming, 650223, China. |
| 推荐引用方式 GB/T 7714 | Cao EH*,Sun XG,Wang XW,et al. Formation and characteristics of an unusual lambda-DNA species[J]. BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,1997,43(3):541-550. |
| APA | Cao EH*.,Sun XG.,Wang XW.,Fang Y.,Bai CL.,...&He YJ.(1997).Formation and characteristics of an unusual lambda-DNA species.BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,43(3),541-550. |
| MLA | Cao EH*,et al."Formation and characteristics of an unusual lambda-DNA species".BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 43.3(1997):541-550. |
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