GRK5 functions as an oncogenic factor in non-small-cell lung cancer

doi:10.1038/s41419-018-0299-1

	GRK5 functions as an oncogenic factor in non-small-cell lung cancer
	Li-Ping Jiang 1,2; Yong-Bin Chen 1; Qiu-Xia Xiong 4; Yong-Chun Zhou 4; Gao-Feng Li 4; Yun-Chao Huang 4; Meng-Ge Wu 4; Qiu-Shuo Shen 1,2; Kun Liu 1,2; Cui-Ping Yang 1
	2018
发表期刊	Cell Death & Disease
期号	9 页码:295
摘要	Lung cancer is the leading cause of cancer-related deaths worldwide, and non-small-cell lung cancer (NSCLC) accounts for about 80% of all cases, which is the major subgroup of lung cancer. G protein-coupled receptor kinase 5 (GRK5) has been demonstrated to play pivotal roles in both development and progression of several pathological conditions including cancer. Here, we found that GRK5 expression was significantly increased in 539 NSCLC cancerous tissues than that in 99 normal non-cancerous tissues by immunohistochemistry analysis; we also showed intensive higher positive staining percentage in female and adenocarcinoma (ADC) NSCLC patients than that in male and squamous cell carcinoma (SCC) patients, respectively. In addition, GRK5 high expression NSCLC patients had a worse overall survival rate than the low expression patients. We provided evidence showing that both the mRNA and protein expression levels of GRK5 were increased in NSCLC cancerous cell lines (GLC-82, SPC-A-1, H520, H838, H358, A549, and H1299) comparing with that in normal human bronchial epithelium cell line (BEAS-2B), and identified many GRK5 mutations in NSCLC cancerous tissues. In addition, we found that depletion of GRK5 inhibited NSCLC cancerous cell proliferation, migration in vitro, and xenograft tumor formation in vivo. Furthermore, GRK5 knockdown promoted cell cycle arrest at G2/M phase and induced cellular apoptosis. In summary, our data reveal an oncogenic role of GRK5 in NSCLC progression, indicating that GRK5 could be used as a new therapeutic target in future.
DOI	10.1038/s41419-018-0299-1
语种	英语
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12261
专题	科研部门_肿瘤信号转导（陈勇彬）
通讯作者	Cui-Ping Yang
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Jiao Chang Dong Lu 32, 650223 Kunming, China. 2.Kunming College of Life Science, University of Chinese Academy of Sciences, 100049 Beijing, China. 3.Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China 4.Kunming Medical University, 650223 Kunming, China
推荐引用方式 GB/T 7714	Li-Ping Jiang,Yong-Bin Chen,Qiu-Xia Xiong,et al. GRK5 functions as an oncogenic factor in non-small-cell lung cancer[J]. Cell Death & Disease,2018(9):295.
APA	Li-Ping Jiang.,Yong-Bin Chen.,Qiu-Xia Xiong.,Yong-Chun Zhou.,Gao-Feng Li.,...&Cui-Ping Yang.(2018).GRK5 functions as an oncogenic factor in non-small-cell lung cancer.Cell Death & Disease(9),295.
MLA	Li-Ping Jiang,et al."GRK5 functions as an oncogenic factor in non-small-cell lung cancer".Cell Death & Disease .9(2018):295.

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