中国科学院昆明动物研究所机构知识库

本论文主要探讨了衰老对猕猴视网膜功能的影响以及性别对衰老猕猴视网膜功能的影响。我们选择了标准视网膜电图五项，即暗适应0.01ERG、暗适应3.0ERG、暗适应3.0震荡电位、明适应3.0ERG、明适应3.0闪烁ERG，和明视负波反应（PhNR）来测量比较了青年雄性组和老年雄性组猕猴视网膜电图的异同，以及老年雄性组和老年雌性组猕猴的视网膜电图的异同。结果显示老年雄性组和青年雄性组猕猴相比，暗适应3.0震荡电位的OS1、OS振幅显著更大，PhNR的a波潜伏期显著变短、PhNR潜伏期显著增大。老年雌性组和老年雄性组猕猴相比，暗适应3.0ERG的a波潜伏期显著增大，明适应3.0ERG的b波振幅和b/a比值显著更小，明适应3.0闪烁ERG的所有波振幅以及振幅的总和均更小，PhNR的a波潜伏期显著增大、b波振幅显著更小、PhNR的a/b比值显著更大。结果表明衰老对猕猴视网膜功能产生了影响：与青年雄性组猕猴相比，老年雄性组猕猴的光感受器功能基本没有差异，只有红光敏感视锥细胞的电信号传导速度更快，双极细胞的功能也没有差异，视网膜内丛状层的反馈抑制活动更明显，神经节细胞的电信号传导速度则变得更慢；性别对衰老猕猴的视网膜功能也具有影响：与老年雄性组猕猴相比，老年雌性组猕猴的明视觉更差，颜色感知能力也更差，视锥细胞的功能更低，视锥细胞驱动的双极细胞、Müller细胞等的数目更少、功能也更低，但暗视觉却没有差异，视杆系统的功能、神经节细胞的数目和功能、视网膜的微循环功能、内丛状层的反馈抑制活动均没有差异。

We evaluated the full field electroretinogram (ERG) to asses age-relate changes in retina function and sex- relate changes in aging retina function in rhesus monkeys. ERG recordings including the ISCEV Standard specifies five responses: (1) Dark-adapted 0.01 ERG; (2) Dark-adapted 3.0 ERG; (3) Dark-adapted 3.0 oscillatory potentials; (4) Light-adapted 3.0 ERG; (5) Light-adapted 3.0 flicker and Photopic Negative Response (PhNR) were performed on twenty-six healthy rhesus monkeys with normal visua function. Changes in ERG properties were found in the older age male group when compared with the younger age male group. (1) Dark-adapted 3.0 oscillatory potentials OS1 and OS were higher; (2) PhNR a-wave implicit times were declined; (3) PhNR implicit times were prolonged. Changes in ERG properties were also found in the older age female group when compared with the older age male group. (1) Dark-adapted 3.0 ERG a-wave implicit times wereprolonged; (2) Light-adapted 3.0 ERG b-wave amplitude were reduced and b/a ratio were decreased; (3) All light-adapted 3.0 flicker amplitudes were reduced; (4) PhNR a-wave implicit times were prolonged , b-wave amplitudes were reduced and PhNR a/b ratio were increased. These results denote that aging had changed retina function: compared with younger age male group, older age male group’s photoreceptors function had no difference, only red-sensitive cone photoreceptors’ electrical signal conduction velocity were faster, bipolar cells’ function also had no difference, the feedback inhibition activity of inner plexiform layer was more evident and the electrical signal conduction velocity of ganglion cells were slower; sex also had effect on aging retina function: compared with old age male group, old age famale group’s light vision and color-perceived function was worse, cones photoreceptors function was worse, the number of cone-driven bipolar cells and Müller cells were less and the function of them were also worse, but dark vision\ rod system\ganglion cells\retina microcirculation between this two groups had no difference.