Infectivity of Zika virus on primary cells support tree shrew as animal model

	Infectivity of Zika virus on primary cells support tree shrew as animal model
	Zhang, L; Shen, ZL; Feng, Y; Li, DQ; Zhang, NN; Deng, YQ; Qi, XP; Sun, XM; Dai, JJ; Yang, CG; Yang, ZF; Qin, CF; Xia, XS
	2019
发表期刊	EMERGING MICROBES & INFECTIONS
ISSN	2222-1751
卷号	8 期号:1 页码:232-241
摘要	Zika virus (ZIKV) is a mosquito-borne flavivirus that caused the public health emergency. Recently, we have proved a novel small animal tree shrew was susceptive to ZIKV infection and presented the most common rash symptoms as ZIKV patients. Here we further cultured the primary cells from different tissues of this animal to determine the tissue tropism of ZIKV infection in vitro. The results showed that the primary cells from tree shrew kidney, lung, liver, skin and aorta were permissive to ZIKV infection and could support viral replication by the detection of viral specific RNA intra- and extra-cells. In comparing, the skin fibroblast and vascular endothelial cells were highly permissive to ZIKV infection with high releasing of active virus particles in supernatants proved by its infectivity in established neonatal mouse model. The expressions of ZIKV envelop and nonstructural protein-1, and the effects and strong immune response of primary tree shrew cells were also detected followed by ZIKV infection. These findings provide powerful in vitro cell-level evidence to support tree shrew as animal model of ZIKV infection and may help to explain the rash manifestations in vivo.
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/12534
专题	科研部门_病原菌感染与宿主免疫机制（齐晓朋）
推荐引用方式 GB/T 7714	Zhang, L,Shen, ZL,Feng, Y,et al. Infectivity of Zika virus on primary cells support tree shrew as animal model[J]. EMERGING MICROBES & INFECTIONS,2019,8(1):232-241.
APA	Zhang, L.,Shen, ZL.,Feng, Y.,Li, DQ.,Zhang, NN.,...&Xia, XS.(2019).Infectivity of Zika virus on primary cells support tree shrew as animal model.EMERGING MICROBES & INFECTIONS,8(1),232-241.
MLA	Zhang, L,et al."Infectivity of Zika virus on primary cells support tree shrew as animal model".EMERGING MICROBES & INFECTIONS 8.1(2019):232-241.

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