Novel contact-kinin inhibitor sylvestin targets thromboinflammation and ameliorates ischemic stroke

	Novel contact-kinin inhibitor sylvestin targets thromboinflammation and ameliorates ischemic stroke
	Zhang, ZY; Shen, CAB; Fang, MQ; Han, YJ; Long, CB; Liu, WH; Yang, M; Liu, M; Zhang, DD; Cao, QQ; Chen, X; Fang, YQ; Lu, QM; Hou, ZL; Li, YX; Liu, ZZ; Lei, X; Ni, HY; Lai, R
	2022
发表期刊	CELLULAR AND MOLECULAR LIFE SCIENCES
ISSN	1420-682X
卷号	79 期号:5
摘要	Ischemic stroke is a leading cause of death and disability worldwide. Increasing evidence indicates that ischemic stroke is a thromboinflammatory disease in which the contact-kinin pathway has a central role by activating pro-coagulant and pro-inflammatory processes. The blocking of distinct members of the contact-kinin pathway is a promising strategy to control ischemic stroke. Here, a plasma kallikrein and active FXII (FXIIa) inhibitor (sylvestin, contained 43 amino acids, with a molecular weight of 4790.4 Da) was first identified from forest leeches (Haemadipsa sylvestris). Testing revealed that sylvestin prolonged activated partial thromboplastin time without affecting prothrombin time. Thromboelastography and clot retraction assays further showed that it extended clotting time in whole blood and inhibited clot retraction in platelet-rich plasma. In addition, sylvestin prevented thrombosis in vivo in FeCl3-induced arterial and carrageenan-induced tail thrombosis models. The potential role of sylvestin in ischemic stroke was evaluated by transient and permanent middle cerebral artery occlusion models. Sylvestin administration profoundly protected mice from ischemic stroke by counteracting intracerebral thrombosis and inflammation. Importantly, sylvestin showed no signs of bleeding tendency. The present study identifies sylvestin is a promising contact-kinin pathway inhibitor that can proffer profound protection from ischemic stroke without increased risk of bleeding.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13255
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
推荐引用方式 GB/T 7714	Zhang, ZY,Shen, CAB,Fang, MQ,et al. Novel contact-kinin inhibitor sylvestin targets thromboinflammation and ameliorates ischemic stroke[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2022,79(5).
APA	Zhang, ZY.,Shen, CAB.,Fang, MQ.,Han, YJ.,Long, CB.,...&Lai, R.(2022).Novel contact-kinin inhibitor sylvestin targets thromboinflammation and ameliorates ischemic stroke.CELLULAR AND MOLECULAR LIFE SCIENCES,79(5).
MLA	Zhang, ZY,et al."Novel contact-kinin inhibitor sylvestin targets thromboinflammation and ameliorates ischemic stroke".CELLULAR AND MOLECULAR LIFE SCIENCES 79.5(2022).

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