Electrochemical sensor for human norovirus based on covalent organic framework/pillararene heterosupramolecular nanocomposites

	Electrochemical sensor for human norovirus based on covalent organic framework/pillararene heterosupramolecular nanocomposites
	Zhao, H; Xie, W; Zhang, RL; Wang, XD; Liu, HF; Li, J; Sha, T; Guo, XS; Li, J; Sun, QM; Zhang, YP; Li, CP
	2022
发表期刊	TALANTA
ISSN	0039-9140
卷号	237
摘要	Noroviruses are the leading cause of acute gastroenteritis and food-borne diseases worldwide. Thus, a rapid, accurate, and easy-to-implement detection method for controlling infection and monitoring progression is urgently needed. In this study, we constructed a novel sandwich-type electrochemical biosensor integrated with two specific recognition elements (aptamer and peptide) for human norovirus (HuNoV). The electrochemical biosensor was fabricated using magnetic covalent organic framework/pillararene heterosupramolecular nano-composites (MB@Apt@WP5A@Au@COF@Fe3O4) as the signal probes. The sensor showed high accuracy and selectivity. The detection method does not need the extraction and amplification of virus nucleic acid and has a short turn-around time. Intriguingly, the proposed biosensor had a limit of detection of 0.84 copy mL(-1) for HuNoV, which was the highest sensitivity among published assays. The proposed biosensor showed higher sensitivity and accuracy compared with immunochromatographic assay in the detection of 98 clinical specimens. The biosensor was capable of determining the predominant infection strain of GII.4 and also GII.3 and achieved 74% selectivity for HuNoV GII group. This study provides a potential method for point-of-care testing and highlights the integrated utilization of Apt and peptide in sensor construction.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13415
专题	科研部门_分子进化与基因组多样性（张亚平）
推荐引用方式 GB/T 7714	Zhao, H,Xie, W,Zhang, RL,et al. Electrochemical sensor for human norovirus based on covalent organic framework/pillararene heterosupramolecular nanocomposites[J]. TALANTA,2022,237.
APA	Zhao, H.,Xie, W.,Zhang, RL.,Wang, XD.,Liu, HF.,...&Li, CP.(2022).Electrochemical sensor for human norovirus based on covalent organic framework/pillararene heterosupramolecular nanocomposites.TALANTA,237.
MLA	Zhao, H,et al."Electrochemical sensor for human norovirus based on covalent organic framework/pillararene heterosupramolecular nanocomposites".TALANTA 237(2022).

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