| Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice |
| Jia, XT; Sun, B; Tu, Q; Qi, HX; Li, L; Liu, XY; Yan, LZ; Dai, HJ; Kong, QP; Tang, CW; Zhao, XD
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| 2021
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| 发表期刊 | PANCREATOLOGY
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| ISSN | 1424-3903
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| 卷号 | 21期号:2页码:418-427 |
| 摘要 | Background: Minor progress in pancreatic cancer treatment and prognosis implies that more reliable animal models are urgently needed to decipher its molecular mechanisms and preclinical research. We recently reported a genetically engineered adult mouse model where Cdkn2b downregulation was required together with Cdkn2a downregulation to inactivate the Rb pathway. Besides, the role of Smad4, which is mutated more frequently than Cdkn2b in human pancreatic cancer, was determined critical on the development of the pancreas tumor by some reports. However, the impact of Smad4 deficiency in combination with PDAC-relevant mutations, such as Cdkn2a when induced in adult pancreas has not been completely elucidated in mice. Methods: Lentiviral delivered oncogene/tumor suppressors in adult pancreas. The development of pancreatic cancer was monitored. Hematoxylin and eosin staining and immunofluorescence were performed for pathological identification of the pancreatic cancer. Real-time polymerase chain reaction, immunofluorescence and western blot were used to test gene expression. Results: Loss of Smad4 could cooperate with alterations of KRAS, Trp53, and Cdkn2a to induce pancreatic cancer in adult mice. The role of Smad4 was mainly in downregulating the expression of Cdkn2b and further inducing phosphorylation of the Rb1 protein. Conclusions: These findings show an essential role of Smad4 deficiency in pancreatic ductal adenocarcinoma (PDAC) formation. This model better recapitulates the adult onset, clonal origin, and genetic alterations in human PDAC and can be simply generated on a large-scale. (C) 2021 IAP and EPC. Published by Elsevier B.V. All rights reserved. |
| 收录类别 | sci
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/13719
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| 专题 | 科研部门_分子人类学（孔庆鹏）
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推荐引用方式
GB/T 7714 |
Jia, XT,Sun, B,Tu, Q,et al. Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice[J]. PANCREATOLOGY,2021,21(2):418-427.
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| APA |
Jia, XT.,Sun, B.,Tu, Q.,Qi, HX.,Li, L.,...&Zhao, XD.(2021).Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice.PANCREATOLOGY,21(2),418-427.
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| MLA |
Jia, XT,et al."Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice".PANCREATOLOGY 21.2(2021):418-427.
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