Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana

	Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana
	Liu, GC; Zhang, R; Hou, QB; He, JW; Dong, ZW; Zhao, RP; Wang, W; Li, XY
	2019
发表期刊	PHOTOCHEMISTRY AND PHOTOBIOLOGY
ISSN	0031-8655
卷号	95 期号:5 页码:1186-1194
摘要	Lamprigera (Lampyridae) is a small genus with only 17 species distributing in Asian countries. Its larviform females and alate males can produce continuously strong yellow-green light at night. However, no luciferase gene was reported for this genus and its subfamily-level phylogenetic position still remains uncertain. Here, we cloned the luciferase gene from one Chinese species, Lamprigera yunnana, by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). This luciferase includes 551 deduced amino acids (AA) with the sequence identity of 71.8-76.8%, 67.5-70.7%, 68.4-75.3%, 77.8% and 59.5% to those from Lampyrinae, Luciolinae, Ototretinae, Cyphonocerinae and Photurinae, respectively. Phylogenetic analyses of deduced AA of luciferases suggest that Lamprigera locates outside Lampyrinae, in which it was originally placed in traditional taxonomy. The luciferase was produced in vitro as recombinant protein, and its biochemical properties were characterized. It possesses significant luminescence activity at pH 7.8, and its K-M for D-luciferin and ATP are 61 mu m and 122 mu m, respectively. It shows the highest activity at 37 degrees C and is completely inactivated at 55 degrees C. It is pH-sensitive with the maximum emission spectrum of 566 nm at pH 7.8. Our data provide new insights into Lamprigera luciferase and its phylogenetic position.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13772
专题	科研部门_遗传多样性基因组学研究组（张国捷）
推荐引用方式 GB/T 7714	Liu, GC,Zhang, R,Hou, QB,et al. Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY,2019,95(5):1186-1194.
APA	Liu, GC.,Zhang, R.,Hou, QB.,He, JW.,Dong, ZW.,...&Li, XY.(2019).Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana.PHOTOCHEMISTRY AND PHOTOBIOLOGY,95(5),1186-1194.
MLA	Liu, GC,et al."Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana".PHOTOCHEMISTRY AND PHOTOBIOLOGY 95.5(2019):1186-1194.

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