Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System

	Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System
	Zhang, M; Wang, P; Luo, RH; Wang, YQ; Li, ZY; Guo, YQ; Yao, YL; Li, MH; Tao, TT; Chen, WW; Han, JB; Liu, HT; Cui, KL; Zhang, X; Zheng, YT; Qin, JH
	2021
发表期刊	ADVANCED SCIENCE
卷号	8 期号:3
摘要	Coronavirus disease 2019 (COVID-19) is a global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The models that can accurately resemble human-relevant responses to viral infection are lacking. Here, a biomimetic human disease model on chip that allows to recapitulate lung injury and immune responses induced by SARS-CoV-2 in vitro at organ level is created. This human alveolar chip reproduce the key features of alveolar-capillary barrier by coculture of human alveolar epithelium, microvascular endothelium, and circulating immune cells under fluidic flow in normal and disease. Upon SARS-CoV-2 infection, the epithelium exhibits higher susceptibility to virus than endothelium. Transcriptional analyses show activated innate immune responses in epithelium and cytokine-dependent pathways in endothelium at day 3 post-infection, revealing the distinctive responses in different cell types. Notably, viral infection causes the immune cell recruitment, endothelium detachment, and increased inflammatory cytokines release, suggesting the crucial role of immune cells involved in alveolar barrier injury and exacerbated inflammation. Treatment with remdesivir can inhibit viral replication and alleviate barrier disruption on chip. This organ chip model can closely mirror human-relevant responses to SARS-CoV-2 infection, which is difficult to be achieved by in vitro models, providing a unique platform for COVID-19 research and drug development.
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/13833
专题	科研部门_分子免疫药理学（郑永唐）
推荐引用方式 GB/T 7714	Zhang, M,Wang, P,Luo, RH,et al. Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System[J]. ADVANCED SCIENCE,2021,8(3).
APA	Zhang, M.,Wang, P.,Luo, RH.,Wang, YQ.,Li, ZY.,...&Qin, JH.(2021).Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System.ADVANCED SCIENCE,8(3).
MLA	Zhang, M,et al."Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System".ADVANCED SCIENCE 8.3(2021).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2023041147.pdf（3521KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文