Electrochemical/colorimetric dual-mode sensing strategy for cardiac troponin I detection based on zirconium nitride functionalized covalent organic frameworks

	Electrochemical/colorimetric dual-mode sensing strategy for cardiac troponin I detection based on zirconium nitride functionalized covalent organic frameworks
	Zhao, H; Zheng, J; Liang, H; Liu, HF; Liu, F; Zhang, YP; Li, CP
	2023
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	391
摘要	Cardiac troponin I (cTnI) is a gold-standard marker for the detection of acute myocardial infarction (AMI). Accurate and timely detection of cTnI is particularly important for AMI diagnosis and therapy. Herein, based on the dual-capacity (electro-catalysis and catalytic degradation) of PtPd@ZrN@COF to toluidine blue (TB), we developed novel electrochemical and enzyme-linked immunosorbent assay (ELISA) methods for ultrasensitive detection of cTnI. In the electrochemical sensor, polyaniline-coated cerium oxide loaded with gold nanoparticles (Au@PANI@CeO2) were used as a substrate platform for immobilizing antibodies (Ab1), and PtPd@ZrN@COF loaded with the affinity peptide (Pep) of cTnI and TB (Pep/TB/PtPd@ZrN@COF) was the probe material for signal amplification of the sensor. Using the 'peptide-cTnI-Ab1 & PRIME; sandwich biosensor, we successfully detected cTnI in a concentration range of 0.00001-50 ng & BULL;mL-1 with an ultralow detection limit (LOD) of 3.3 fg & BULL;mL-1. In addition, a sandwich ELISA was constructed by 96-well plate using Pep/PtPd@ZrN@COF as the probe molecule for cTnI detection, showing a detection range of 0.00001-5 ng & BULL;mL-1 and a LOD of 3.3 fg & BULL;mL-1. Both detection methods presented excellent accuracy, selectivity, reproducibility, and stability, providing a potential application in clinical diagnosis of AMI.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14007
专题	科研部门_分子进化与基因组多样性（张亚平）
推荐引用方式 GB/T 7714	Zhao, H,Zheng, J,Liang, H,et al. Electrochemical/colorimetric dual-mode sensing strategy for cardiac troponin I detection based on zirconium nitride functionalized covalent organic frameworks[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,391.
APA	Zhao, H.,Zheng, J.,Liang, H.,Liu, HF.,Liu, F.,...&Li, CP.(2023).Electrochemical/colorimetric dual-mode sensing strategy for cardiac troponin I detection based on zirconium nitride functionalized covalent organic frameworks.SENSORS AND ACTUATORS B-CHEMICAL,391.
MLA	Zhao, H,et al."Electrochemical/colorimetric dual-mode sensing strategy for cardiac troponin I detection based on zirconium nitride functionalized covalent organic frameworks".SENSORS AND ACTUATORS B-CHEMICAL 391(2023).

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