| A ?peptide-target-aptamer? electrochemical biosensor for norovirus detection using a black phosphorous nanosheet@Ti3C2-Mxene nanohybrid and magnetic covalent organic framework |
| Liu, HF; Ma, SY; Ning, GB; Zhang, RL; Liang, H; Liu, F; Xiao, L; Guo, LJ; Zhang, YP ; Li, CP; Zhao, H
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| 2023
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| 发表期刊 | TALANTA
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| ISSN | 0039-9140
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| 卷号 | 258 |
| 摘要 | Norovirus (NoV) is a major foodborne pathogen responsible for acute gastroenteritis epidemics, and establishing a robust detection method for the timely identification and monitoring of NoV contamination is of great significance. In this study, a peptide-target-aptamer sandwich electrochemical biosensor for NoV was fabricated using Au@BP@Ti3C2-MXene and magnetic Au@ZnFe2O4@COF nanocomposites. The response currents of the electrochemical biosensor were proportional to the NoV concentrations ranging from 0.01-105 copies/mL with a detection limit (LOD) of 0.003 copies/mL (S/N = 3). To our best knowledge, this LOD was the lowest among published assays to date, due to the specific recognition of the affinity peptide and aptamer for NoV and the outstanding catalytic activity of nanomaterials. Furthermore, the biosensor showed excellent selectivity, antiinterference performance, and satisfactory stability. The NoV concentrations in simulative food matrixes were successfully detected using the constructed biosensor. Meanwhile, NoV in stool samples was also successfully quantified without complex pretreatment. The designed biosensor had the potential to detect NoV (even at a low level) in foods, clinical samples, and environmental samples, providing a new method for NoV detection in food safety and diagnosing foodborne pathogens. |
| 收录类别 | SCI
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/14009
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| 专题 | 科研部门_分子进化与基因组多样性（张亚平）
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推荐引用方式
GB/T 7714 |
Liu, HF,Ma, SY,Ning, GB,et al. A ?peptide-target-aptamer? electrochemical biosensor for norovirus detection using a black phosphorous nanosheet@Ti3C2-Mxene nanohybrid and magnetic covalent organic framework[J]. TALANTA,2023,258.
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| APA |
Liu, HF.,Ma, SY.,Ning, GB.,Zhang, RL.,Liang, H.,...&Zhao, H.(2023).A ?peptide-target-aptamer? electrochemical biosensor for norovirus detection using a black phosphorous nanosheet@Ti3C2-Mxene nanohybrid and magnetic covalent organic framework.TALANTA,258.
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| MLA |
Liu, HF,et al."A ?peptide-target-aptamer? electrochemical biosensor for norovirus detection using a black phosphorous nanosheet@Ti3C2-Mxene nanohybrid and magnetic covalent organic framework".TALANTA 258(2023).
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