| Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis |
| Bi, R; Pan, LN; Dai, H; Sun, CL; Li, C; Lin, HJ; Xie, LP; Ma, HX; Li, L; Xie, H; Guo, K; Hou, CH; Yao, YG ; Chen, LN; Zheng, P
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| 2024
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| 发表期刊 | NUCLEIC ACIDS RESEARCH
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| ISSN | 0305-1048
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| 卷号 | 52期号:22页码:13644-13664 |
| 摘要 | Spermatogonial stem cells (SSCs) play crucial roles in the preservation of male fertility. However, successful ex vivo expansion of authentic human SSCs remains elusive due to the inadequate understanding of SSC homeostasis regulation. Using rhesus monkeys (Macaca mulatta) as a representative model, we characterized SSCs and progenitor subsets through single-cell RNA sequencing using a cell-specific network approach. We also profiled chromatin status and major histone modifications (H3K4me1, H3K4me3, H3K27ac, H3K27me3 and H3K9me3), and subsequently mapped promoters and active enhancers in TSPAN33(+) putative SSCs. Comparing the epigenetic changes between fresh TSPAN33(+) cells and cultured TSPAN33(+) cells (resembling progenitors), we identified the regulatory elements with higher activity in SSCs, and the potential transcription factors and signaling pathways implicated in SSC regulation. Specifically, TGF-beta signaling is activated in monkey putative SSCs. We provided evidence supporting its role in promoting self-renewal of monkey SSCs in culture. Overall, this study outlines the epigenetic landscapes of monkey SSCs and provides clues for optimization of the culture condition for primate SSCs expansion. [GRAPHICS] . |
| 收录类别 | SCI
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| 语种 | 英语
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/14533
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| 专题 | 科研部门_哺乳动物胚胎发育（郑萍）
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推荐引用方式
GB/T 7714 |
Bi, R,Pan, LN,Dai, H,et al. Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis[J]. NUCLEIC ACIDS RESEARCH,2024,52(22):13644-13664.
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| APA |
Bi, R.,Pan, LN.,Dai, H.,Sun, CL.,Li, C.,...&Zheng, P.(2024).Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis.NUCLEIC ACIDS RESEARCH,52(22),13644-13664.
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| MLA |
Bi, R,et al."Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis".NUCLEIC ACIDS RESEARCH 52.22(2024):13644-13664.
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