Single-molecule chromatin profiling reveals cell type-specific A/B compartment alteration and multi-enhancer transcriptional coordination

	Single-molecule chromatin profiling reveals cell type-specific A/B compartment alteration and multi-enhancer transcriptional coordination
	Liu, LR; Zhong, JY; Bai, X; Ye, CL; Hou, CH; Ding, JJ; Chi, W; Xiao, CL; Niu, LJ
	2026
发表期刊	J. Genet. Genomics
ISSN	1673-8527
卷号	53 期号:3 页码:522-536
摘要	In eukaryotic organisms, the three-dimensional organization and epigenomic landscape of chromatin are fundamental to the regulation of gene expression. Previous studies have provided significant insights into CpG methylation, chromatin accessibility, and the dynamics of 3D architecture. However, a systematic delineation of how these epigenomic features regulate transcriptional activity remains limited. In this study, we develop nanoCAM-seq, a single-molecule sequencing technique designed to simultaneously profile higher-order chromatin interactions, chromatin accessibility, and endogenous CpG methylation. This approach provides an integrative view of chromatin features associated with cis-regulatory elements and reveals their coordinated dynamics during transitions of A/B compartments. Single-molecule analyses using nanoCAM-seq further reveal that promoters characterized by low CpG methylation and high chromatin accessibility more frequently interact with multiple enhancers. Collectively, our findings establish nanoCAM-seq as a powerful approach for resolving the coordinated dynamics of chromatin architecture and epigenetic modifications, offering critical insights into the regulatory mechanisms underlying gene expression. Copyright (c) 2025, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/14745
专题	科研部门_进化发育与四维基因调控（候春晖）
推荐引用方式 GB/T 7714	Liu, LR,Zhong, JY,Bai, X,et al. Single-molecule chromatin profiling reveals cell type-specific A/B compartment alteration and multi-enhancer transcriptional coordination[J]. J. Genet. Genomics,2026,53(3):522-536.
APA	Liu, LR.,Zhong, JY.,Bai, X.,Ye, CL.,Hou, CH.,...&Niu, LJ.(2026).Single-molecule chromatin profiling reveals cell type-specific A/B compartment alteration and multi-enhancer transcriptional coordination.J. Genet. Genomics,53(3),522-536.
MLA	Liu, LR,et al."Single-molecule chromatin profiling reveals cell type-specific A/B compartment alteration and multi-enhancer transcriptional coordination".J. Genet. Genomics 53.3(2026):522-536.

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