Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting

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	Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting
	Nie WH*1; Wang JH 1; Su WT 1; Wang YX 1; Yang FT 2; whnie@mail.kiz.ac.cn
	2009
发表期刊	CHROMOSOME RESEARCH
ISSN	0967-3849
卷号	17 期号:3 页码:321-329
合作性质	其它
摘要	The Chinese pangolin (Manis pentadactyla), a representative species of the order Pholidota, has been enlisted in the mammalian whole-genome sequencing project mainly because of its phylogenetic importance. Previous studies showed that the diploid number of M. pentadactyla could vary from 2n = 36 to 42. To further characterize the genome organization of M. pentadactyla and to elucidate chromosomal mechanism underlying the karyotype diversity of Pholidota, we flow-sorted the chromosomes of 2n = 40 M. pentadactyla, and generated a set of chromosome-specific probes by DOP-PCR amplification of flow-sorted chromosomes. A comparative chromosome map between M. pentadactyla and the Malayan pangolin (Manis javanica, 2n = 38), as well as between human and M. pentadactyla, was established by chromosome painting for the first time. Our results demonstrate that seven Robertsonian rearrangements, together with considerable variations in the quantity of heterochromatin and in the number of nucleolar organizer regions (NORs) differentiate the karyotypes of 2n = 38 M. javanica and 2n = 40 M. pentadactyla. Moreover, we confirm that the M. javanica Y chromosome bears one NOR. Comparison of human homologous segment associations found in the genomes of M. javanica and M. pentadactyla revealed seven shared associations (HSA 1q/11, 2p/5, 2q/10q, 4p+q/20, 5/13, 6/19p and 8q/10p) that could constitute the potential Pholidota-specific signature rearrangements.
关键词	Pangolin Manis Fluorescence In-situ Hybridization Chromosome Painting Chromosome Rearrangement Karyotype Cytogenetics
资助者	This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). ; This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). ; This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). ; This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293).
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收录类别	SCI
语种	英语
资助者	This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). ; This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). ; This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). ; This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2059
专题	遗传资源与进化国家重点实验室_保护遗传学(细胞库) 遗传资源与进化国家重点实验室其他
通讯作者	whnie@mail.kiz.ac.cn
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China 2.Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
推荐引用方式 GB/T 7714	Nie WH*,Wang JH,Su WT,et al. Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting[J]. CHROMOSOME RESEARCH,2009,17(3):321-329.
APA	Nie WH*,Wang JH,Su WT,Wang YX,Yang FT,&whnie@mail.kiz.ac.cn.(2009).Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting.CHROMOSOME RESEARCH,17(3),321-329.
MLA	Nie WH*,et al."Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting".CHROMOSOME RESEARCH 17.3(2009):321-329.

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