A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product

	A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product
	Lai R; Liu H; Lee WH; Zhang Y*; zhangy@mail.kiz.ac.cn
	2001
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	286 期号:2 页码:259-263
合作性质	其它
摘要	Amphibian skin contains rich bradykinin-related peptides, but the mode of biosynthesis of these peptides is unknown. In the present study, a novel bradykinin-related peptide, termed bombinakinin M, was purified from skin secretions of the Chinese red belly toad Bombina maxima. Its primary sequence was established as DLPKINRKGPRPPGFSPFR that comprises bradykinin extended from its N-terminus by a 10-residue segment DLPKINRKGP. The cDNA structure of bombinakinin M was found to contain a coding region of 624 nucleotides. The encoded precursor of bombinakinin M is composed of a signal peptide, an acidic peptide, six 100% identical copies of a 28-amino-acid peptide unit including bombinakinin M plus a spacer peptide. The sequence of bombinakinin M is preceded by a single basic residue (arginine), which represents the site of cleavage for releasing of mature bombinakinin M. This is the first cDNA cloning of bradykinin-related peptides from amphibian skin. The unique cDNA structure encoding bombinakinin M suggests that the generation modes of bradykinin-related peptides in amphibian skin and in mammalian blood system are different.
关键词	Bradykinin Amphibian Bombinakinin m Bombina Maxima Cdna Clone Kallikrein-kinin System
资助者	This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission. ; This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission. ; This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission. ; This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission.
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission. ; This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission. ; This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission. ; This work was supported by the grants of “STZ98-3-01,” “Western Light Project,” and “Tenth Five Plan” preresearch project from Chi- nese Academy of Sciences and a grant from the Yunnan Science and Technology Commission.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2461
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China
推荐引用方式 GB/T 7714	Lai R,Liu H,Lee WH,et al. A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,286(2):259-263.
APA	Lai R,Liu H,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2001).A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,286(2),259-263.
MLA	Lai R,et al."A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 286.2(2001):259-263.

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