Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain

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	Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain
	Guo J*1; Chen YH 2; Zhou KY 1; Li JY 3; jguo58@yahoo.com
	2005
发表期刊	BIOTECHNIC & HISTOCHEMISTRY
ISSN	0884-6812
卷号	80 期号:1 页码:31-34
合作性质	其它
摘要	Previous investigations have proved that diplomonads have primitive cell nuclei and lack a nucleolus. We determined the distribution of ribosomal DNA (rDNA) in diplomonad nuclei that lacked a nucleolus. Giardia lamblia was used as the experimental organism with Euglena gracilis as the control. The distribution of rDNA was demonstrated indirectly by the modified Ag-I silver technique that can indicate specifically the nucleolus organizing region (NOR) by both light and electron microscopy. In ultrathin sections of silver stained Euglena cells, all silver grains were concentrated in the fibrosa of the nucleolus, while no silver grains were found in the cytoplasm, nucleoplasm, condensed chromosomes or pars granulosa of the nucleus. In the silver stained Giardia cells, no nucleolus was found, but a few silver grains were scattered in the nucleus. This suggests that the rDNA of Giardia does not form an NOR-like structure and that its nucleus is in a primitive state.
关键词	Giardia Lamblia Nor Nucleolus Organizing Region Silver Staining
资助者	This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. ; This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. ; This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. ; This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094.
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收录类别	SCI
语种	英语
资助者	This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. ; This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. ; This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094. ; This work was partly sup- ported by a National Nature Science Foundation of China grant No. 39270094.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2479
专题	其他细胞与分子进化开放实验室
通讯作者	jguo58@yahoo.com
作者单位	1.College of Life Science, Nanjing Normal University, Nanjing, 210097, P.R. China 2.Department of Histology and Embryology, Nanjing Military Medical College of the Second Military Medical University, Nanjing 3.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P.R. China
推荐引用方式 GB/T 7714	Guo J*,Chen YH,Zhou KY,et al. Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain[J]. BIOTECHNIC & HISTOCHEMISTRY,2005,80(1):31-34.
APA	Guo J*,Chen YH,Zhou KY,Li JY,&jguo58@yahoo.com.(2005).Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain.BIOTECHNIC & HISTOCHEMISTRY,80(1),31-34.
MLA	Guo J*,et al."Distribution of rDNA in the nucleus of Giardia lamblia - Detection by Ag-I silver stain".BIOTECHNIC & HISTOCHEMISTRY 80.1(2005):31-34.

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