A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis

	A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis
	Yu D 1,2; Sheng ZG 1,2; Xu XQ 1; Li JX 1; Yang HL 1; Liu ZG 3; Rees HH 4; Lai R*1; rlai@mail.kiz.ac.cn
	2006
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	27 期号:1 页码:31-35
合作性质	其它
摘要	A novel antimicrobial peptide named as ixosin was isolated from the salivary glands of the hard tick, Ixodes sinensis, by gel filtration, ion exchange chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as GLHKVMREVLGYERNSYKKFFLR by Edman degradation and its molecular weight was 2870.5 analyzed by fast atom bombardment (FAB) mass spectrometry. This is the first antimicrobial peptide from ticks that lacks cysteine in its primary structure. The cDNA encoding ixosin was cloned by cDNA library screening. The predicted protein from the cDNA sequence composed of 79 amino acids including mature ixosin. Purified ixosin exerted its antimicrobial activities against bacteria and fungi. No similarity was found by BLAST search to any database entries and, thus, our findings describe a novel antimicrobial peptide.
关键词	Ticks Antimicrobial Peptide Ixodes Sinensis Salivary Gland
资助者	This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101). ; This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101). ; This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101). ; This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101).
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收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101). ; This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101). ; This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101). ; This work was supported by the Chinese National Natural Science Foundation (30200044/C01040101).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2495
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Toxicology Department, 32 East Jiao Chang Road, Kunming, Yunnan 650223, PR China 2.Department of Life Science & Technology, Changshu Institute of Technology, Changshu 215500, PR China 3.College of Life Sciences, Shenzhen University, Shenzhen 518060, PR China 4.School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, UK
推荐引用方式 GB/T 7714	Yu D,Sheng ZG,Xu XQ,et al. A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis[J]. PEPTIDES,2006,27(1):31-35.
APA	Yu D.,Sheng ZG.,Xu XQ.,Li JX.,Yang HL.,...&rlai@mail.kiz.ac.cn.(2006).A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis.PEPTIDES,27(1),31-35.
MLA	Yu D,et al."A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis".PEPTIDES 27.1(2006):31-35.

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