A bactericidal homodimeric phospholipases A(2) from Bungarus fasciatus venom

	A bactericidal homodimeric phospholipases A(2) from Bungarus fasciatus venom
	Xu CH 1; Ma DY 2,4; Yu HN 3; Li ZJ 1; Liang JG 1; Lin GQ 1; Zhang Y 2; Lai R*1,2; rlai72@njau.edu.cn
	2007
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	28 期号:5 页码:969-973
合作性质	其它
摘要	Group IIA secretory phospholipases A(2) (sPLA(2)-II) is generally known to display potent grampositive bactericidal activity, while group IA sPLA(2) (sPLA(2)-I) reportedly is not. In this work, a novel sPLA(2)-I named BFPA was identified from Bungarus fasciatus venom, and its antimicrobial activity was studied as well. The amino acid sequence of the venomous protein precursor was 145-amino acid in length, and contained a predicted 27-amino acid signal peptide and a 118-amino acid mature protein. Unlike the well-known sPLA(2)-IS, which have 14 half-cysteines forming 7 intramolecular disulfide bridges, BFPA possesses 15 half-cysteines. The additional cysteine might contribute to the formation of an intermolecular disulfide bridge of the homodimeric protein. In the biological activities assays, BFPA displayed the activities of anticoagulation and bactericidal against Escherichia coli and Staphylococcus aureus. This study is the first report about gram-positive bactericidal activity of sPLA(2)-I.
关键词	Pla(2) Bungarus Fasciatus Antibacterial Snake Venom
资助者	This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422).
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收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422). ; This work was supported by the Chinese National Natural Science Foundation (30570360, 30670456), Yunnan Natural Science Foundation (2005C0054M, 2006C0011Z), the grant 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China, and Jiangsu Natural Science Foundation (BK2005422).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2511
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室科研部门_生物毒素与人类疾病（张云）
通讯作者	rlai72@njau.edu.cn
作者单位	1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 2.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.College of Life Sciences, School of Hebei Normal University, Shijiazhuang 050016, Hebei, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Xu CH,Ma DY,Yu HN,et al. A bactericidal homodimeric phospholipases A(2) from Bungarus fasciatus venom[J]. PEPTIDES,2007,28(5):969-973.
APA	Xu CH.,Ma DY.,Yu HN.,Li ZJ.,Liang JG.,...&rlai72@njau.edu.cn.(2007).A bactericidal homodimeric phospholipases A(2) from Bungarus fasciatus venom.PEPTIDES,28(5),969-973.
MLA	Xu CH,et al."A bactericidal homodimeric phospholipases A(2) from Bungarus fasciatus venom".PEPTIDES 28.5(2007):969-973.

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