A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami

	A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami
	Che QL 1; Zhou Y 1; Yang HL 2; Li JX 2,3; Xu XQ 2,3; Lai R*1,2; rlai72@njau.edu.cn
	2008
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	29 期号:4 页码:529-535
合作性质	其它
摘要	A novel antimicrobial peptide named odorranain-NR was identified from skin secretions of the diskless odorous frog, Odorrana grahami. It is composed of 23 amino acids with an amino acid sequence of GLLSGILGAGKHIVCGLTGCAKA. Odorranain-NR was classified into a novel family of antimicrobial peptide although it shared similarity with amphibian antimicrobial peptide family of nigrocin. Odorranain-NR has an unusual intramolecular disulfide-bridged hexapeptide segment that is different from the intramolecular disulfide-bridged heptapeptide segment at the C-terminal end of nigrocins. Furthermore, the -AKA fragment at the C-terminal of odorranain-NR is also different from nigrocins. Three different cDNAs encoding two odorranain-NR precursors and only one mature odorranain-NR was cloned from the cDNA library of the skin of O. grahami. This peptide showed antimicrobial activities against tested microorganisms except Escherichia coli (ATCC25922). Its antimicrobial mechanisms were investigated by transmission electron microcopy. odorranain-NR exerted its antimicrobial functions by various means depending on different microorganisms.
关键词	Amphibian Antimicrobial Peptide Odorrana Grahami Innate Immunity
资助者	This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China.
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收录类别	SCI
语种	英语
资助者	This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China. ; This work was supported by Yunnan Natural Science Foundation (2006C0011Z), 2006BAD06B07 from the Ministry of Science and Technology of the People’s Republic of China.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2543
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai72@njau.edu.cn
作者单位	1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 2.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Che QL,Zhou Y,Yang HL,et al. A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami[J]. PEPTIDES,2008,29(4):529-535.
APA	Che QL.,Zhou Y.,Yang HL.,Li JX.,Xu XQ.,...&rlai72@njau.edu.cn.(2008).A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami.PEPTIDES,29(4),529-535.
MLA	Che QL,et al."A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami".PEPTIDES 29.4(2008):529-535.

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