A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith)

	A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith)
	Yang HL 1,3; Xu XQ 1,3; Ma DY 1,3; Zhang KY 2; Lai R*1,2; rlai@mail.kiz.ac.cn
	2008
发表期刊	TOXICON
ISSN	0041-0101
卷号	51 期号:2 页码:289-296
合作性质	其它
摘要	Wasp is an impor tant venomous animal that can induce human fatalities. Aortic thrombosis and cerebral infarction are major clinical symptoms after massive wasp stings but the reason leading to the envenomation manifestation is still not known. In this paper, a toxin protein is purified and characterized by Sephadex G-75 gel filtration, CM-Sephadex C-25 cationic exchange and fast protein liquid chromatography (FPLC) from the venom of the wasp, Vespa magnifica (Smith). This protein, named magnifin, contains phospholipase-like activity and induces platelet aggregation. The cDNA encoding magnifin is cloned from the venom sac cDNA library of the wasp. The predicted protein was deduced from the cDNA with a sequence composed of 337 amino acid residues. Magnifin is very similar to other phospholipase A(1) (PLA(1)), especially to other wasp allergen PLA(1). Magnifin can activate platelet aggregation and induce thrombosis in vivo. The current results proved that PLA(1) in wasp venom could be contributable to aortic thrombosis after massive wasp stings.
关键词	Wasp Venom Vespa Magnifica Phospholipase A1 Platelet Activator Aortic Thrombosis
资助者	This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences. ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences. ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences. ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences.
URL	查看原文
收录类别	SCI
语种	英语
资助者	This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences. ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences. ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences. ; This work was supported by Jiangsu Natural Science Foundation (BK2005422), 2006 BAD 06B07 from the Ministry of Science and Technology of the People’s Republic of China, and KSCX2- YW-R-20 and KSCX2-YW-G-024 from the Chi- nese Academy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2551
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratories of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Yang HL,Xu XQ,Ma DY,et al. A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith)[J]. TOXICON,2008,51(2):289-296.
APA	Yang HL,Xu XQ,Ma DY,Zhang KY,Lai R*,&rlai@mail.kiz.ac.cn.(2008).A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith).TOXICON,51(2),289-296.
MLA	Yang HL,et al."A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith)".TOXICON 51.2(2008):289-296.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
200851289.pdf（374KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文