| Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri | |
| Lee WH1,2; Zhuang QY1; Zhang Y*1; zhangy@mail.kiz.ac.cn | |
| 2003 | |
| 发表期刊 | TOXICON
 |
| ISSN | 0041-0101 |
| 卷号 | 41期号:7页码:765-772 |
| 合作性质 | 其它 |
| 摘要 | A blood coagulation factor IX-binding protein (TSV-FIX-BP) was isolated from the snake venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-FIX-BP showed a single band with an apparent molecular weight of 23,000 under non-reducing conditions, and two distinct bands with apparent molecular weights of 14,800 and 14,000 under reducing conditions. cDNA clones containing the coding sequences of TSV-FIX-BP were isolated and sequenced to determine the structure of the precursors of TSV-FIX-BP subunits. The deduced amino acid sequences of two subunits of TSV-FIX-BP were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. TSV-FIX-BP was a non-enzymatic C-type lectin-like anti-coagulant. The anti-coagulant activity of TSV-FIX-BP was mainly caused by its dose dependent interaction with blood coagulation factor IX but not with blood coagulation factor X. |
| 关键词 | Snake Venom C-type Lectin-like Protein Coagulation Fix-binding Protein Trimeresurus Stejnegeri Cdna Sequence |
| 资助者 | This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). ; This work was supported by the grants of ‘Western Light Project’ and ‘Tenth Five Years Plan’ pre-research project from the Chinese Academy of Sciences; and grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (99C0085M, 2001C0061M). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/2653 |
| 专题 | 科研部门_生物毒素与人类疾病（张云） |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, People’s Republic of China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Lee WH,Zhuang QY,Zhang Y*,et al. Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri[J]. TOXICON,2003,41(7):765-772. |
| APA | Lee WH,Zhuang QY,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2003).Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri.TOXICON,41(7),765-772. |
| MLA | Lee WH,et al."Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri".TOXICON 41.7(2003):765-772. |
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| 200341765.pdf（467KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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