Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi

	Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi
	Zhao Y 1,2; Jin Y 1; Wei SS 1,2; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2005
发表期刊	TOXICON
ISSN	0041-0101
卷号	46 期号:6 页码:635-640
摘要	Amphibian skin secretions contain many bioactive compounds. In the present work, an irreversible serine protease inhibitor, termed baserpin, was purified for the first time from the skin secretions of toad Bufo andrewsi by successive ion-exchange and gel-filtration chromatography. Baserpin is a single chain glycoprotein, with an apparent molecular weight of about 60 kDa in SDS-PAGE. Baserpin is an irreversible inhibitor and effectively inhibits the catalytic activity of trypsin, chymotrypsin and elastase. SDS-stable baserpin-trypsin complex could be seen in SDS-PAGE indicates that it possibly belongs to the serpin superfamily. According to the association rates determined, baserpin is a potent inhibitor of bovine trypsin (4.6 x 10(6) M(-1) s(-1)), bovine chymotrypsin (8.9 x 10(6) M(-1) s(-1)) and porcine elastase (6.8 x 10(6) M(-1) s(-1)), whereas it shows no inhibitory effect on thrombin. The N-terminal sequence of baserpin is HTQYPDILIAKPXDK, which shows no similarity with other known serine protease inhibitors.
关键词	Serine Protease Inhibitor Bufo Andrewsi Skin Secretions
资助者	National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M).
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收录类别	SCI
语种	英语
资助者	National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M). ; National Natural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003C0066M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2667
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Zhao Y,Jin Y,Wei SS,et al. Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi[J]. TOXICON,2005,46(6):635-640.
APA	Zhao Y,Jin Y,Wei SS,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2005).Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi.TOXICON,46(6),635-640.
MLA	Zhao Y,et al."Purification and characterization of an irreversible serine protease inhibitor from skin secretions of Bufo andrewsi".TOXICON 46.6(2005):635-640.

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