| Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions | |
| Zhang J1,2; Zhang Yong1,2; Wan SG1,2; Wei SS1,2; Lee WH1; Zhang Y*1; zhangy@mail.kiz.ac.cn | |
| 2005 | |
| 发表期刊 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| ISSN | 0006-291X |
| 卷号 | 330期号:4页码:1027-1033 |
| 合作性质 | 其它 |
| 摘要 | In mammals, trefoil factor family (TFF) proteins are involved in mucosal maintenance and repair, and they are also implicated in tumor suppression and cancer progression. A novel two domain TFF protein from frog Bombina maxima skin secretions (Bm-TFF2) has been purified and cloned. It activated human platelets in a dose-dependent manner and activation of integrin alpha(IIb)beta(3) was involved. Aspirin and apyrase did not largely reduce platelet response to Bm-TFF2 (a 30% inhibition), indicating that the aggregation is not substantially dependent on ADP and thromboxane A2 autocrine feedback. Elimination of external Ca(2+) with EGTA did not influence the platelet aggregation induced by Bm-TFF2, meanwhile a strong calcium signal (cytoplasmic Ca(2+) release) was detected, suggesting that activation of phospholipase C (PLC) is involved. Subsequent immunoblotting revealed that, unlike in platelets activated by stejnulxin (a glycoprotein VI agonist), PLCgamma2 was not phosphorylated in platelets activated by Bm-TFF2. FITC-labeled Bm-TFF2 bound to platelet membranes. Bm-TFF2 is the first TFF protein reported to possess human platelet activation activity. |
| 关键词 | Trefoil Factor Platelet Activation Sequence Amphibian Bombina Bm-tff2 |
| 资助者 | This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). ; This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). ; This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). ; This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). ; This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). ; This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). ; This work was supported by the grants of ‘‘Western Light’’ Projects from The Chinese Academy of Sciences to Drs. Zhang and Lee; and grants from National Nat- ural Science Foundation (30170195, 30470380) and Yunnan Science and Technology Commission (2003 C0066M). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/2677 |
| 专题 | 科研部门_生物毒素与人类疾病（张云） |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Zhang J,Zhang Yong,Wan SG,et al. Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,330(4):1027-1033. |
| APA | Zhang J.,Zhang Yong.,Wan SG.,Wei SS.,Lee WH.,...&zhangy@mail.kiz.ac.cn.(2005).Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,330(4),1027-1033. |
| MLA | Zhang J,et al."Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 330.4(2005):1027-1033. |
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| 20053301027.pdf（467KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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