Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland

	Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland
	Wan SG 1,2; Jin Y 1; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2006
发表期刊	TOXICON
ISSN	0041-0101
卷号	47 期号:4 页码:465-472
合作性质	其它
摘要	Hemorrhagic toxins are widely distributed in viperid and crotalid snake venoms. Envenomation of Trimeresurus stejnegeri, a member of Crotalidae family, caused potent systemic and local hemorrhage. Up to now, there is no report on hemorrhage toxins from this venom. In this work, we cloned two cDNAs of P-III metalloproteinase precursors, designated as stejnihagin-A and stejnihagin-B, respectively, from T. stejnegeri venom gland. Both cDNAs encode an opening reading frame of 600 amino acid residues, containing a signal sequence, a proprotein domain, a metalloproteinase domain, a disintegrin-like domain and a cystetine-rich domain. Sequence analysis suggested that these two sequences shared highest similarity to the hemorrhagic toxin HR1b from T. flavoviridis. Aligning the deduced mature protein sequences of stejnihagin-A and stejnihagin-B with other snake venom metalloproteinases (SVMPs), we observed that stejnihagin-A and stejnihagin-B, together with HR1b shared the common cysteinyl residue at the position 100 in the metalloproteinase domain. In combination with the phylogenetic analysis, we presumed that stejnihagin-A, stejnihagin-B and HR1b might constitute a novel subclass of P-III SVMPs, named P-IIIc.
关键词	Cdna Cloning Snake Venom Metalloproteinase Trimeresurs Stejnegeri
资助者	This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M).
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M). ; This work was supported by the grants of ‘Western Light’ Projects from The Chinese Academy of Sciences to Dr Zhang and Dr Lee; and grants from National Natural Science Foundation (30470380, 30570359) and Yunnan Science and Technology Commission (2003C0066M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2689
专题	科研部门_生物毒素与人类疾病（张云）
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Wan SG,Jin Y,Lee WH,et al. Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland[J]. TOXICON,2006,47(4):465-472.
APA	Wan SG,Jin Y,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2006).Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland.TOXICON,47(4),465-472.
MLA	Wan SG,et al."Cloning of two novel P-III class metalloproteinases from Trimeresurus stejnegeri venom gland".TOXICON 47.4(2006):465-472.

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