Molecular characterization of L-amino acid oxidase from king cobra venom

	Molecular characterization of L-amino acid oxidase from king cobra venom
	Jin Y 1; Lee WH 1; Zeng L 1,2; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2007
发表期刊	TOXICON
ISSN	0041-0101
卷号	50 期号:4 页码:479-489
合作性质	其它
摘要	An L-amino acid oxidase from Ophiophagus hannah snake venom (Oh-LAAO) was purified by successive gel filtration, ion-exchange and heparin chromatography. Oh-LAAO did not induce platelet aggregation; however, it had potent inhibitory activity on platelet aggregation induced by ADP and U46619, but showed no effect on platelet aggregation induced by thrombin, mucetin, ristocetin and stejnulxin. By RT-PCR and 5'-RACE methods, the complete Oh-LAAO cDNA was cloned from the venom gland total RNA preparations. The cDNA sequence contains an open-reading frame (ORF) of 1476-bp, which encodes a protein of 491 amino acids comprising a signal peptide of 25 amino acids and 466-residue mature protein. The predicted protein sequence of Oh-LAAO was confirmed by N-terminal and trypsin-digested internal peptides sequencing together with peptide mass fingerprinting. cDNAs encoding for ORF of LAAOs from Bungarus fasciatus and B. multicinctus were cloned and reported in this study. In addition, partial cDNA encoding for Naja atra LAAO was also reported. Oh-LAAO shared approximately 50% protein sequence identity with other known snake venom LAAOs. Phylogenetic analysis indicated that Oh-LAAO is evolutionary distant to other snake venom LAAOs.
关键词	l -amino Acid Oxidase Elapid Snake Venom Platelet Aggregation Sequence
资助者	The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. ; The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. ; The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. ; The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood.
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收录类别	SCI
语种	英语
资助者	The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. ; The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. ; The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood. ; The work was supported by grants from Yunnan Natural Science Foundation (2005PY01-23, 2003C0066M, 2006C0047Q), grants from National Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and grants of ‘‘Western Light’’ Projects. We also acknowledge Yunnan Blood Center for providing human blood.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2705
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Jin Y,Lee WH,Zeng L,et al. Molecular characterization of L-amino acid oxidase from king cobra venom[J]. TOXICON,2007,50(4):479-489.
APA	Jin Y,Lee WH,Zeng L,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2007).Molecular characterization of L-amino acid oxidase from king cobra venom.TOXICON,50(4),479-489.
MLA	Jin Y,et al."Molecular characterization of L-amino acid oxidase from king cobra venom".TOXICON 50.4(2007):479-489.

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