Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom

	Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
	He YY 1,2; Liu SB 1,2; Lee WH 1,3; Qian JQ 1,2,3,4; Zhang Y*1,3; zhangy@mail.kiz.ac.cn
	2008
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	29 期号:10 页码:1692-1699
合作性质	其它
摘要	Snake venom Kunitz/BPTI members are good tools for understanding of structure-functional relationship between serine proteases and their inhibitors. A novel dual Kunitz/BPTI serine proteinase inhibitor named OH-TCI (trypsin- and chymotrypsin-dual inhibitor from Ophiophagus hannah) was isolated from king cobra venom by three chromatographic steps of gel filtration, trypsin affinity and reverse phase HPLC. OH-TCI is composed of 58 amino acid residues with a molecular mass of 6339 Da. Successful expression of OH-TCI was performed as the maltose-binding fusion protein in E. coli DH5 alpha . Much different from Oh11-1, the purified native and recombinant OH-TCI both had strong inhibitory activities against trypsin and chymotrypsin although the sequence identity (74.1%) between them is very high. The inhibitor constants (K-i) of recombinant OH-TCI were 3.91 x 10(-7) and 8.46 x 10(-8) M for trypsin and chymotrypsin, respectively. To our knowledge, it was the first report of Kunitz/BPTI serine proteinase inhibitor from snake venom that had equivalent trypsin and chymotrypsin inhibitory activities.
关键词	Serine Proteinase Inhibitor Trypsin Inhibitor Chymotrypsin Inhibitor Snake Venom Kunitz/bpti Family Polypeptide King Cobra
资助者	This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. ; This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. ; This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. ; This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences.
URL	查看原文
收录类别	SCI
语种	英语
资助者	This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. ; This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. ; This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences. ; This work was supported by grants from Chinese National Natural Science Foundation (30630014, 30570359 and 30670412) and grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’, ‘‘Western Light’’ Projects from the Chinese Academy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2709
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3.Kunming Medical College, Kunming, Yunnan 650032, China 4.Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650031, China
推荐引用方式 GB/T 7714	He YY,Liu SB,Lee WH,et al. Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom[J]. PEPTIDES,2008,29(10):1692-1699.
APA	He YY,Liu SB,Lee WH,Qian JQ,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2008).Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom.PEPTIDES,29(10),1692-1699.
MLA	He YY,et al."Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom".PEPTIDES 29.10(2008):1692-1699.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2008291692.pdf（967KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文