In vitro cell-mediated and complement=mediated cytotoxicity to murine testicular cells

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	In vitro cell-mediated and complement=mediated cytotoxicity to murine testicular cells
	Ben KL 1; Alexander NJ 2; Huso N 2
	1986
发表期刊	American Journal of Reproductive Immunology and Microbiology
期号	12 页码:33-37
合作性质	其它
摘要	Male BALB/c mice at 8 to 14 weeks of age were divided into three groups: group 1 was immunized with an emulsion of testicular cells (TCs) (10(7)/mouse), complete Freund's adjuvant (CFA), and extract of Bordetella pertussis (BP); group 2 was given CFA and BP injections; and group 3 was given sterile saline injections. Suspensions of TCs and spleen cells (SCs) from each mouse were prepared 4 weeks after the first immunization for a cytotoxic T lymphocyte (CTL) assay. For the antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent complement-mediated cytotoxicity (ACC) assays, TCs and SCs were prepared from normal male and female mice, respectively. Targets were labeled with Na251 Cr0(4). The interactions of targets (TCs) and effectors (SCs) were conducted at 32.5 degrees C (for CTL, ADCC, and ACC assays) or 37 degrees C (for ACC assay). In the CTL assay, SCs from group 1 and group 2 caused significantly more killing than those from group 3. Specific cytotoxicity in the ADCC assay was only detected in the serum (maximum specific lysis 47.65%) of one mouse. No other cytotoxicity was detectable in 61 serum samples from group 1 (n = 25), group 2 (n = 17), and group 3 (n = 19). In the ACC assay, no significant specific cytolysis was found at different incubation temperatures (32.5 and 37.0 degrees C) in 44 serum samples from the three groups. These results suggest that CTLs are important in the pathogenesis of experimental allergic orchitis (EAO). Adjuvant alone, probably because of breakdown of the blood-testis barrier, causes significant T lymphocyte cytotoxicity to TCs.(ABSTRACT TRUNCATED AT 250 WORDS)
关键词	Cytotoxic t Lymphocyte Experimental Allergic Orchitis Antibody-dependent Complement-mediated Cytotoxicity Adjuvant
收录类别	其他
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/277
专题	其他
作者单位	1.Kunming Institute of Zoology,Kunming,China 2.Oregon Regional Primate Research Center, Beaverton, Oregon
推荐引用方式 GB/T 7714	Ben KL,Alexander NJ,Huso N. In vitro cell-mediated and complement=mediated cytotoxicity to murine testicular cells[J]. American Journal of Reproductive Immunology and Microbiology,1986(12):33-37.
APA	Ben KL,Alexander NJ,&Huso N.(1986).In vitro cell-mediated and complement=mediated cytotoxicity to murine testicular cells.American Journal of Reproductive Immunology and Microbiology(12),33-37.
MLA	Ben KL,et al."In vitro cell-mediated and complement=mediated cytotoxicity to murine testicular cells".American Journal of Reproductive Immunology and Microbiology .12(1986):33-37.

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