Comparative study of three short-chain neurotoxins from the venom of Naja kaouthia (Yunnan, China)

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	Comparative study of three short-chain neurotoxins from the venom of Naja kaouthia (Yunnan, China)
	Lu QM; Meng QX; Li DS; Zhu SW; Jia YH; Xiong YL; Wang WY*; wangwy@mail.kiz.ac.cn
	2002
发表期刊	JOURNAL OF NATURAL TOXINS
ISSN	1058-8108
卷号	11 期号:3 页码:221-229
合作性质	其它
摘要	Three short-chain neurotoxins named NT-I, NT-II, and NT-III were purified from the venom of Naja kaouthia, a snake distributed throughout the south of Yunnan province, China, by a series of chromatographic steps, including an FPLC Resource S column. Their molecular weights, determined by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS, were 6952.19 Da, 6854.92 Da, and 6828.80 Da, respectively. NT-I consisted of 62 amino acid residues, and the other two consisted of 61 amino acid residues, including 8 cysteines. After hydrolysis by endoproteinase Glu-C, their primary sequences were determined. A test of their activities demonstrated that they effectively inhibited muscle contractions induced by electric stimulation. Furthermore, the extent of inhibition caused by NT-II and NT-III was less than that of NT-I. The IC(50)s were 0.04 mug/ml, 0.20 mug/ml, and 0.23 mug/ml for NT-I, NT-II, and NT-III, respectively. Compared with NT-II and NT-III, the higher activity of NT-I may be a result of the amino acid residue substitution Ile36 to Arg36.
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2797
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	wangwy@mail.kiz.ac.cn
作者单位	Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxicol, Kunming 650223, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Lu QM,Meng QX,Li DS,et al. Comparative study of three short-chain neurotoxins from the venom of Naja kaouthia (Yunnan, China)[J]. JOURNAL OF NATURAL TOXINS,2002,11(3):221-229.
APA	Lu QM.,Meng QX.,Li DS.,Zhu SW.,Jia YH.,...&wangwy@mail.kiz.ac.cn.(2002).Comparative study of three short-chain neurotoxins from the venom of Naja kaouthia (Yunnan, China).JOURNAL OF NATURAL TOXINS,11(3),221-229.
MLA	Lu QM,et al."Comparative study of three short-chain neurotoxins from the venom of Naja kaouthia (Yunnan, China)".JOURNAL OF NATURAL TOXINS 11.3(2002):221-229.

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