A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin

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	A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin
	Chen RQ 1,2; Jin Y 1; Wu JB 1,2; Zhou XD 1,2; Li DS 1; Lu QM 1; Wang WY 1; Xiong YL*1; chenrunqiang@hotmail.com
	2004
发表期刊	TOXICON
ISSN	0041-0101
卷号	44 期号:3 页码:281-287
合作性质	其它
摘要	A hemorrhagic proteinase, jerdohagin, was purified from Trimeresurus jerdonii venom by gel filtration and ion-exchange chromatographies. It was a single chain polypeptide with an apparent molecular weight of 96 kDa as estimated by SDS-PAGE under the non-reducing and reducing conditions. Internal peptide sequencing indicated that it consisted of metalloproteinase, disintegrin-like and cysteine-rich domains and belonged to the class III snake venom metalloproteinases (class P-III SVMPs). Like other typical metalloproteinases, hemorrhagic activities of jerdohagin were completely inhibited by EDTA, but not by PMSF. Jerdohagin preferentially degraded alpha-chain of human fibrinogen. Interestingly, jerdohagin did not activate human prothrombin, whereas it cleaved human prothrombin and fragment F1 of activated human prothrombin.
关键词	Hemorrhage Svmp Trimeresurus Jerdonii Fibrinogenolytic Activity Activation Fragment F1
资助者	This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). ; This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). ; This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). ; This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M).
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收录类别	SCI
语种	英语
资助者	This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). ; This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). ; This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). ; This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2807
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	chenrunqiang@hotmail.com
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Chen RQ,Jin Y,Wu JB,et al. A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin[J]. TOXICON,2004,44(3):281-287.
APA	Chen RQ.,Jin Y.,Wu JB.,Zhou XD.,Li DS.,...&chenrunqiang@hotmail.com.(2004).A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin.TOXICON,44(3),281-287.
MLA	Chen RQ,et al."A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin".TOXICON 44.3(2004):281-287.

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