源真核生物蓝氏贾第虫核分裂的初步观察

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	源真核生物蓝氏贾第虫核分裂的初步观察
其他题名	The Preliminary Study on The Nuclear Division of The Archezoan,Giardia lamblia
	沈剑钊 1; 李靖炎 2; 卢思奇 1
	1996
发表期刊	动物学研究
ISSN	0254-5853
卷号	17 期号:3 页码:295-299
摘要	首次对蓝氏贾第虫(Giardlia lamblia)的核分裂作了电镜观察, 未能在分裂着的核中见到纺缍体或纺缍体微管。以秋水仙素实验和微管蛋白作免疫荧光检查后, 似乎意味着其核分裂方式乃是前有丝分裂性质的, 并作了讨论。
其他摘要	Diplomonads seem to be the most primitive archezoa.In literature,almost all the studies on diplomonad nuclear division were accomplished under light microscope,Considerring the primitiveness of diplomonad nucleus,we investigated the nuclear division of cultivated Giardia lamblia with routine electron-microscopy preliminarily.Nuclear envelope was found to be preseved during the whole division process.Spindle or spindle microtubules were never found within dividing nucleus or cytoplasm.No condensed metaphase chromosomes were observed,although heterochromatin could occationally be seen both in dividing nucleus and in interphase nucleus.If there is surely no spinde participating the division process,then colchcine should have no influence on this process.Our experiments showed that various concentrations of colchicine (0.1-20 μg/ml),certainly had no effect on the cell division of Giardia.Immuno-fluorescent microscopical detection was done with anti-tubulin antibody and it was found that no tubulin or tubulin-made structure existed in the interphase nucleus and dividing nucleus,while cytoplasm and flagella gave strong positive reaction.The resuts described above suggested that there is no spindle formed during the nuclear division of Giardia.The possible significance of this phenomenon in the origin and evolution of mitosis was discussed.
部门归属	其他
关键词	贾第虫核分裂纺锤体微管蛋白秋水仙素
资助者	国家自然科学基金 ; 国家自然科学基金
URL	查看原文
收录类别	其他
语种	中文
资助者	国家自然科学基金 ; 国家自然科学基金
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3451
专题	其他细胞与分子进化开放实验室
作者单位	1.首都医科大学寄生虫学教研室 2.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223
推荐引用方式 GB/T 7714	沈剑钊,李靖炎,卢思奇. 源真核生物蓝氏贾第虫核分裂的初步观察[J]. 动物学研究,1996,17(3):295-299.
APA	沈剑钊,李靖炎,&卢思奇.(1996).源真核生物蓝氏贾第虫核分裂的初步观察.动物学研究,17(3),295-299.
MLA	沈剑钊,et al."源真核生物蓝氏贾第虫核分裂的初步观察".动物学研究 17.3(1996):295-299.

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1996173295.pdf（415KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文