A Highly Active Anticomplement Factor from the Venom of Naja kaouthia

	A Highly Active Anticomplement Factor from the Venom of Naja kaouthia
其他题名	一个高活性的盂加拉眼镜蛇毒抗补体因子
	Sun QY; Lu QM; Wang WY; Xiong YL*; sunqy@hotmail. com
	2001
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	0582-9879
卷号	33 期号:5 页码:483-488
合作性质	其它
摘要	A highly active anticomplement factor (cobra venom factor) from the venom of Naja kaouthia in South Yunnan, China was isolated by sequential column chromatography (SP-Sephadex-C25, Q Sepharose HP and Sephadex G-150). It displays strong anticomplement activities in vitro and in vivo, and has anticomplement activity of 1 515 u per mg. The purified anticomplement factor was homogeneous on SDS-PAGE with a molecular weight of 149 kD. It is composed of three polypeptide chains which are connected by disulfide bonds. The molecular weights of the three chains were determined to be 65. 4 kD (alpha -chain), 52. 1 kD (beta -chain), and 35. 5 kD (gamma -chain). The gamma -chain displayed size heterogeneity, and two bands could be clearly detected in gels. All polypeptide chains were stained with periodate-Schiff reagent, suggesting the presence of carbohydrate. Neutral sugar and sialic acid were determined to be 1. 78 % and 0. 38 %, respectively. The isoelectric point of this anticomplement factor was 6. 2. Its amino acid composition was analyzed and N-terminal amino acid sequence of each polypeptide chain was determined.
其他摘要	用离子交换层析和分子筛从云南南部产的盂加拉眼镜蛇（Naja kaouthia)蛇毒中分离到一个高活性的抗补体因子（anticomplement factor)。它表现出较强的体内、体外抗补体活性，其抗补体活性的比活力为1515u/mg，纯化的抗补体因子在聚丙烯酰胺凝胶电泳中呈现一条带，经SDS-PAGE，确定其全分子量为149kD，还原性SDS-PAGE表明，它由3条多肽链共价结合而成，3条多肽链分子量分别为65.4kD、52.1kD和35.5kD。最小的一条多肽链在还原条件下呈现多态性，一般可见两条带（35.5kD和33.7kD)。过碘酸席夫试剂染色表明，其3条多肽链均含有糖。定量测定表明中性糖含量为1.78%，唾液酸含量为0.38%，其等电点为6.2。对其氨基酸组成分析表明它含有较多的酸性氨基酸，对其3条多肽链的N末端氨基酸序列进行了测定。
关键词	Anticomplement Factor Complement Venom
资助者	This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) ; This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) ; This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) ; This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z)
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收录类别	其他
语种	英语
资助者	This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) ; This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) ; This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) ; This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z)
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3795
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	sunqy@hotmail. com
作者单位	Kunming Institute of Zoology , the Chinese Academy of Sciences , Kunming 650223 , China
推荐引用方式 GB/T 7714	Sun QY,Lu QM,Wang WY,et al. A Highly Active Anticomplement Factor from the Venom of Naja kaouthia[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):483-488.
APA	Sun QY,Lu QM,Wang WY,Xiong YL*,&sunqy@hotmail. com.(2001).A Highly Active Anticomplement Factor from the Venom of Naja kaouthia.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),483-488.
MLA	Sun QY,et al."A Highly Active Anticomplement Factor from the Venom of Naja kaouthia".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):483-488.

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