The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg

	The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg
	Chen DY 1; Sun QY 1; Liu JL 1; Li GP 1; Lian L 1; Wang MK 1; Han ZM 1; Song XF 1; Li JS 1; Sun Q 1; Chen YC 2; Zhang YP3 ; Ding B 3
	1999
发表期刊	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN	1006-9305
卷号	42 期号:4 页码:346-353
产权排序	The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg
合作性质	其它
摘要	The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of interspecies embryos by transferring them into enucleated rabbit eggs. All the three kinds of somatic cells were able to reprogram in rabbit ooplasm and support early embryo development, of which mammary gland cells were proven to be the Lest, followed by uterus epithelial cells and skeletal muscle cells. The experiments showed that direct injection of mammary gland cell into enucleated rabbit ooplasm, combined with in vivo development in ligated rabbit oviduct, achieved higher blastocyst development than in vitro culture after the somatic cell was injected into the perivitelline space and fused with the enucleated egg by electrical stimulation. The chromosome analysis demonstrated that the genetic materials in reconstructed blastocyst cells were the same as that in panda somatic cells. In addition, giant panda mitochondrial DNA (mtDNA) was shown to exist in the interspecies reconstructed blastocyst. The data suggest that (i) the ability of ooplasm to dedifferentiate somatic cells is not species-specific; (ii) there is compatibility between interspecies somatic nucleus and ooplasm during early development of the reconstructed egg.
关键词	Giant Panda Rabbit Interspecies Cloning Dedifferentiation Totipotency
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3915
专题	科研部门_分子进化与基因组多样性（张亚平）
作者单位	1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2.Fuzhou Giant Panda Research Center, Fuzhou 350001, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
推荐引用方式 GB/T 7714	Chen DY,Sun QY,Liu JL,et al. The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1999,42(4):346-353.
APA	Chen DY.,Sun QY.,Liu JL.,Li GP.,Lian L.,...&Ding B.(1999).The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,42(4),346-353.
MLA	Chen DY,et al."The giant panda (Ailurapoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 42.4(1999):346-353.

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