Identification of Sarcocystis hominis-like (Protozoa : Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences

	Identification of Sarcocystis hominis-like (Protozoa : Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences
	Yang ZQ 2,3; Zuo YX1; Ding B 3; Chen XW 1; Luo J 3; Zhang YP*3
	2001
发表期刊	JOURNAL OF PARASITOLOGY
ISSN	0022-3395
卷号	87 期号:4 页码:934-937
合作性质	其它
摘要	DNA templates were extracted from isolates of Sarcocystis hominis-like cysts collected from cattle and water buffalo, as well as from Sarcocystis fusiformis cysts and Sarcocystis suihominis cysts. The 18S rRNA genes were amplified using DNA from a single cyst as the templates. Approximately 1,367-1,440 bp sequences were obtained. The sequence difference in isolates of Sarcocystis hominis-like cysts from water buffaloes. and isolates of S. hominis cysts from cattle were very low, only about 0.1%, much lower than the lowest value (1.7%) among different species. Combined with their morphological structure, these sequence data indicate that the 4 isolates from cattle and water buffalo might be the same species. i.e.. S. hominis, suggesting that both cattle and water buffalo may serve as the intermediate hosts for this parasite. Apparently, this is the first report using a single cyst to do such work and is a useful way to distinguish the Sarcocystis cyst in an intermediate host that may be simultaneously infected by several different Sarcocystis species.
资助者	The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program. ; The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program. ; The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program. ; The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program.
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收录类别	SCI
语种	英语
资助者	The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program. ; The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program. ; The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program. ; The present study was funded by the Yunnan teaching com- mittee foundation for science, no. 0011001, the Kunming Medical College foundation for science, and the College foundation for the Ph.D. Program.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4013
专题	科研部门_分子进化与基因组多样性（张亚平）细胞与分子进化开放实验室
作者单位	1.Biology Department, Yunnan University, Kunming, Yunnan 650091 China 2.Parasitology Department, Kunming Medical College, Kunming, Yunnan 650031 China 3.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
推荐引用方式 GB/T 7714	Yang ZQ,Zuo YX*,Ding B,et al. Identification of Sarcocystis hominis-like (Protozoa : Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences[J]. JOURNAL OF PARASITOLOGY,2001,87(4):934-937.
APA	Yang ZQ,Zuo YX*,Ding B,Chen XW,Luo J,&Zhang YP.(2001).Identification of Sarcocystis hominis-like (Protozoa : Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences.JOURNAL OF PARASITOLOGY,87(4),934-937.
MLA	Yang ZQ,et al."Identification of Sarcocystis hominis-like (Protozoa : Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences".JOURNAL OF PARASITOLOGY 87.4(2001):934-937.

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