Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis

	Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis
	Miao YC 1; Su JR1; Zhang ZJ 1; Li H 2; Luo J 3,4; Zhang YP*2,3
	2008
发表期刊	CONSERVATION GENETICS
ISSN	1566-0621
卷号	9 期号:6 页码:1683-1685
合作性质	其它
摘要	Eleven polymorphic microsatellite loci were characterized for the endangered conifer Taxus yunnanensis. Eight loci were isolated through SSR-anchored PCR, one locus was developed by cross-species amplification tests, while the last two loci were obtained from cross-species microsatellite sequences available in GenBank. Variability of these markers was tested in 48 individuals collected from its main distribution range in Southwest China. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity varied from 0.000 to 0.625 and from 0.062 to 0.853, respectively. Ten of these eleven loci were significantly deviated from Hardy-Weinberg expectation, except TS07 which showed a distinct heterozygote excess. The availability of these new polymorphic microsatellite markers will provide an ideal marker system for detailed population genetics studies in T. yunnanensis and potentially also for closely related species.
关键词	Ssr-anchored Pcr Microsatellite Marker Ssrs Taxus Yunnanensis
资助者	This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z)
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收录类别	SCI
语种	英语
资助者	This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z) ; This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of For- estry (RIRI200703Z)
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4277
专题	科研部门_分子进化与基因组多样性（张亚平）遗传资源与进化国家重点实验室
作者单位	1.Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Bailongshi, Kunming 650224, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, China 4.School of Life Science, Yunnan University, Kunming 650091, China
推荐引用方式 GB/T 7714	Miao YC,Su JR*,Zhang ZJ,et al. Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis[J]. CONSERVATION GENETICS,2008,9(6):1683-1685.
APA	Miao YC,Su JR*,Zhang ZJ,Li H,Luo J,&Zhang YP.(2008).Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis.CONSERVATION GENETICS,9(6),1683-1685.
MLA	Miao YC,et al."Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis".CONSERVATION GENETICS 9.6(2008):1683-1685.

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