Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes

KIZ OpenIR > 基因起源组

	Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes
	Li X 1,2; Zhao L 1,2; Jiang HF 1,2; Wang W*1; wwang@mail.kiz.ac.cn
	2009
发表期刊	JOURNAL OF MOLECULAR EVOLUTION
ISSN	0022-2844
卷号	68 期号:1 页码:56-65
合作性质	其它
摘要	Chimeric RNAs have been reported in varieties of organisms and are conventionally thought to be produced by trans-splicing of two or more distinct transcripts. Here, we conducted a large-scale search for chimeric RNAs in the budding yeast, fruit fly, mouse, and human. Thousands of chimeric transcripts were identified in these organisms except in yeast, in which five chimeric RNAs were observed. RT-PCR experiments for a sample of yeast and fly chimeric transcripts using specific primers show that about one-third of these chimeric RNAs can be reproduced. The results suggest that at least a considerable amount of chimeric RNAs is unlikely from aberrant transcription or splicing, and thus formation of chimeric RNAs is probably a widespread process and can greatly contribute to the complexity of the transcriptome and proteome of organisms. However, only a small fraction (< 20%) of these chimeric RNAs has GU-AG at the junction sequences which fits the classical trans-splicing model. In contrast, we observed that about half of the chimeric RNAs have short homologous sequences (SHSs) at the junction sites of the source sequences. Our sequence mutation experiments in yeast showed that disruption of SHSs resulted in the disappearance of the corresponding chimeric RNAs, suggesting that SHSs are essential for generating this kind of chimeric RNA. In addition to the classical trans-splicing model, we propose a new model, the transcriptional slippage model, to explain the generation of those chimeric RNAs synthesized from templates with SHSs.
关键词	Chimeric Rnas Short Homologous Sequences Trans-splicing Transcriptional Slippage Complexity Of Transcriptome
资助者	This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. ; This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. ; This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. ; This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W.
收录类别	SCI
语种	英语
资助者	This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. ; This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. ; This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W. ; This work was supported by a CAS-Max Planck Society Fellowship, a NSFC key grant (No. 30430400), and a 973 Program grant (No. 2007CB815703-5) to W.W.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4853
专题	基因起源组遗传资源与进化国家重点实验室
通讯作者	wwang@mail.kiz.ac.cn
作者单位	1.CAS-Max Planck Junior Research Group on Evolutionary Genomics, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming 650223, China 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Li X,Zhao L,Jiang HF,et al. Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes[J]. JOURNAL OF MOLECULAR EVOLUTION,2009,68(1):56-65.
APA	Li X,Zhao L,Jiang HF,Wang W*,&wwang@mail.kiz.ac.cn.(2009).Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes.JOURNAL OF MOLECULAR EVOLUTION,68(1),56-65.
MLA	Li X,et al."Short Homologous Sequences Are Strongly Associated with the Generation of Chimeric RNAs in Eukaryotes".JOURNAL OF MOLECULAR EVOLUTION 68.1(2009):56-65.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
200968156.pdf（356KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文