Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus

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	Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus
其他题名	湖南烙铁头蛇毒中一个新的舒缓激肽增强肽(TmF)
	Jia YH 1,2; Li DS 1; Zhu SW 1; Zhang LY 1; Ding LS 3; Wang WY 1; Xiong YL*1; wangwy@mail.kiz.ac.cn
	2003
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	0582-9879
卷号	35 期号:7 页码:619-623
合作性质	其它
摘要	A novel bradykinin-potentiating peptide (BPP), designated as TmF, has been purified to homogeneity from the venom of Trimeresurus mucrosquamatus by 70% cold methanol extraction, Sephadex G-15 gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). The amino acid sequence of TmF was determined to be pGlu-Gly-Arg-Pro-Leu-Gly-Pro-Pro-Ile-Pro-Pro (pGlu denotes pyroglutamic acid), which shared high homology with other BPPs. The molecular mass of TmF was 1.1107 kD as determinated by electrospray ionization-mass spectrometry (ESI-MS), which was in accordance with the calculated value of 1.1106 kD. The potentiating unit of TmF to bradykinin-induced (BK-induced) contraction on the guinea-pig ileum in vitro was (1.13 +/-0.3) unit (mg/L), and TmF (5.0 x10(-4) mg/kg) increased the pressure-lowering-effect of bradykinin (5.0 x10(-5 )mg/kg) with approximate descent value of (14 +/-2) mmHg. In addition, TmF inhibited the conversion of angiotensin I to angiotensin II, 2 x10(-3) mg of TmF caused 50% inhibition (IC(50)) of angiotensin- converting enzyme (ACE) hydrolyzing activity to bradykinin.
其他摘要	通过 70 %冷甲醇抽提、SephadexG 15分子筛和反相高效液相色谱C1 8层析 ,从湖南产烙铁头蛇毒 (Trimeresurusmucrosquamatus)冻干粉中纯化得到一个新的舒缓激肽增强肽 (BPP) ,命名为TmF。该小肽的氨基酸序列为pGlu Gly Arg Pro Leu Gly Pro Pro Ile Pro Pro (pGlu表示焦谷氨酸 )。序列结果分析表明 ,TmF和已经分离得到的BPPs有很高的序列同源性。MSI MS测定其分子量为 1.110 7kD。TmF的生物学活性和药理学活性检测的结果表明 ,它增强舒缓激肽 (BK) ( 1mg L)诱导的离体豚鼠回肠纵行肌收缩的活性为 ( 1.13± 0 .3)单位 (mg L) ;TmF ( 5 .0× 10 - 4mg kg)可以增强约 ( 14± 2 )mmHg的由BK( 5 .0× 10 - 5mg kg)诱导的舒张压下降 ;在抑制剂试验中 ,不同剂量的TmF和 5× 10 - 2 mg的血管紧张素转化酶保温 30min ,结果表明大约2 .0× 10 - 3mg的TmF表现出对ACE水解活性的半数抑制率 (IC50 )。
关键词	Angiotensin-converting Enzyme Bradykinin Bradykinin-potentiating Peptide Tmf
资助者	This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q) ; This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q) ; This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q) ; This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q)
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收录类别	其他
语种	英语
资助者	This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q) ; This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q) ; This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q) ; This work was supported by a grant from the Yunnan Y outh Science Foundation of China (No. 1999C0019Q)
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5275
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	wangwy@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology , Kunming Institute of Zoology , the Chinese Academy of Sciences , Kunming 650223 , China 2.Graduate School of the Chinese Academy of Sciences , Beijing 100009 , China 3.Chengdu Institute of Biology , the Chinese Academy of Sciences , Chengdu 610041 , China
推荐引用方式 GB/T 7714	Jia YH,Li DS,Zhu SW,et al. Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(7):619-623.
APA	Jia YH.,Li DS.,Zhu SW.,Zhang LY.,Ding LS.,...&wangwy@mail.kiz.ac.cn.(2003).Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(7),619-623.
MLA	Jia YH,et al."Characterization of a new bradykinin-potentiating peptide (TmF) from Trimeresurus mucrosquamatus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.7(2003):619-623.

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