Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom

	Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom
其他题名	烙铁头蛇毒L-2氨基酸氧化酶的分离、纯化及其生物学活性
	Wei JF 1,2; Wei Q 1; Lu QM 1; Tai H ; Jin Y 1; Wang WY 1; Xiong YL*1; wangwy@mail. kiz.ac.cn
	2003
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	0582-9879
卷号	35 期号:3 页码:219-224
合作性质	其它
摘要	An L-amino acid oxidase (TM-LAO) from the venom of Hunan Trimeresurus mucrosquamatus was purified to homogeneity by three steps including DEAE Sephadex A-50 ion-exchange chromatography, Sephadex G-75 gel filtration and Resource Q ion-exchange chromatography. TM-LAO is composed of two identical subunits with a molecular weight of 55 kD by SDS-polyacrylamide gel electrophoresis. The molecular weight was different with that of LAO purified from the same species distributed in Taiwan that was 70 kD. The 24 N-terminal amino acid sequence of TM-LAO is ADNKNPLEECFRETNYEEFLEIAR, which shares high similarity with other Viperid snake venom LAOs and has moderate similarity with Elapid snake venom LAOs. Further studies found that TM-LAO inhibited the growth of E. coli, S. aurues and B. dysenteriae. TM-LAO also showed cytotoxicity and platelet aggregation activity. All the biological activities were eliminated by catalase, a H(2)O(2) scavenger. It was shown that these biological effects were possibly due to the formation of H(2)O(2) produced by TM-LAO.
其他摘要	通过 DEAE Sephadex A 2 50 阴离子交换柱 , Sephadex G 2 75 分子筛 , Resourse Q 阴离子交换柱三步层析从湖南产的烙铁头蛇毒中分离、纯化得到一个 L 2氨基酸氧化酶(TM2 LAO) , 它由两个非共价的亚基组成 , 每个亚基的分子量为 55 kD 。与台湾产的烙铁头蛇毒 L 2氨基酸氧化酶分子量 (70 kD) 不同。 TM 2 LAO 的 N 末端氨基酸序列是 ADNK NPLEECFRETNYEEFLEIAR , 与报道的蝰科的 L 2氨基酸氧化酶的相似性比眼镜蛇科的要高。 TM 2 LAO 能抑制大肠杆菌、金黄色葡萄球菌和痢疾杆菌的生长 , 杀死肿瘤细胞以及诱导血小板聚集。这些活性能被过氧化氢酶所抑制 , 说明 TM 2 LAO 生理学功能主要是通过酶反应产生的过氧化氢(H2 O 2 ) 介导的。
关键词	L-amino Acid Oxidase Trimeresurus Mucrosquamatus Antibacterial Activity Cytotoxicity Platelet Aggregation
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5283
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	wangwy@mail. kiz.ac.cn
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxicol, Kunming 650223, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wei JF,Wei Q,Lu QM,et al. Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(3):219-224.
APA	Wei JF.,Wei Q.,Lu QM.,Tai H.,Jin Y.,...&wangwy@mail. kiz.ac.cn.(2003).Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(3),219-224.
MLA	Wei JF,et al."Purification, characterization and biological activity of an L-amino acid oxidase from Trimeresurus mucrosquamatus venom".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.3(2003):219-224.

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